H3K4me3 breadth is linked to cell identity and transcriptional consistency

Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that spread more broadly over genes in a given cell type preferentially mark genes that are essential for the identity and fu...

Full description

Saved in:

Bibliographic Details
Published in:	Cell Vol. 158; no. 3; p. 673
Main Authors:	Benayoun, Bérénice A, Pollina, Elizabeth A, Ucar, Duygu, Mahmoudi, Salah, Karra, Kalpana, Wong, Edith D, Devarajan, Keerthana, Daugherty, Aaron C, Kundaje, Anshul B, Mancini, Elena, Hitz, Benjamin C, Gupta, Rakhi, Rando, Thomas A, Baker, Julie C, Snyder, Michael P, Cherry, J Michael, Brunet, Anne
Format:	Journal Article
Language:	English
Published:	United States 31.07.2014
Subjects:	Animals Artificial Intelligence Cells - metabolism Genomics Histone Code Histones - metabolism Humans Lysine - metabolism Methylation Mice, Inbred C57BL Neural Stem Cells - metabolism RNA Polymerase II - metabolism Transcription, Genetic
ISSN:	1097-4172, 1097-4172
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that spread more broadly over genes in a given cell type preferentially mark genes that are essential for the identity and function of that cell type. Using the broadest H3K4me3 domains as a discovery tool in neural progenitor cells, we identify novel regulators of these cells. Machine learning models reveal that the broadest H3K4me3 domains represent a distinct entity, characterized by increased marks of elongation. The broadest H3K4me3 domains also have more paused polymerase at their promoters, suggesting a unique transcriptional output. Indeed, genes marked by the broadest H3K4me3 domains exhibit enhanced transcriptional consistency and [corrected] increased transcriptional levels, and perturbation of H3K4me3 breadth leads to changes in transcriptional consistency. Thus, H3K4me3 breadth contains information that could ensure transcriptional precision at key cell identity/function genes.
AbstractList	Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that spread more broadly over genes in a given cell type preferentially mark genes that are essential for the identity and function of that cell type. Using the broadest H3K4me3 domains as a discovery tool in neural progenitor cells, we identify novel regulators of these cells. Machine learning models reveal that the broadest H3K4me3 domains represent a distinct entity, characterized by increased marks of elongation. The broadest H3K4me3 domains also have more paused polymerase at their promoters, suggesting a unique transcriptional output. Indeed, genes marked by the broadest H3K4me3 domains exhibit enhanced transcriptional consistency and [corrected] increased transcriptional levels, and perturbation of H3K4me3 breadth leads to changes in transcriptional consistency. Thus, H3K4me3 breadth contains information that could ensure transcriptional precision at key cell identity/function genes.Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that spread more broadly over genes in a given cell type preferentially mark genes that are essential for the identity and function of that cell type. Using the broadest H3K4me3 domains as a discovery tool in neural progenitor cells, we identify novel regulators of these cells. Machine learning models reveal that the broadest H3K4me3 domains represent a distinct entity, characterized by increased marks of elongation. The broadest H3K4me3 domains also have more paused polymerase at their promoters, suggesting a unique transcriptional output. Indeed, genes marked by the broadest H3K4me3 domains exhibit enhanced transcriptional consistency and [corrected] increased transcriptional levels, and perturbation of H3K4me3 breadth leads to changes in transcriptional consistency. Thus, H3K4me3 breadth contains information that could ensure transcriptional precision at key cell identity/function genes. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that spread more broadly over genes in a given cell type preferentially mark genes that are essential for the identity and function of that cell type. Using the broadest H3K4me3 domains as a discovery tool in neural progenitor cells, we identify novel regulators of these cells. Machine learning models reveal that the broadest H3K4me3 domains represent a distinct entity, characterized by increased marks of elongation. The broadest H3K4me3 domains also have more paused polymerase at their promoters, suggesting a unique transcriptional output. Indeed, genes marked by the broadest H3K4me3 domains exhibit enhanced transcriptional consistency and [corrected] increased transcriptional levels, and perturbation of H3K4me3 breadth leads to changes in transcriptional consistency. Thus, H3K4me3 breadth contains information that could ensure transcriptional precision at key cell identity/function genes.
Author	Devarajan, Keerthana Benayoun, Bérénice A Gupta, Rakhi Wong, Edith D Snyder, Michael P Brunet, Anne Mahmoudi, Salah Ucar, Duygu Baker, Julie C Mancini, Elena Cherry, J Michael Karra, Kalpana Pollina, Elizabeth A Kundaje, Anshul B Daugherty, Aaron C Hitz, Benjamin C Rando, Thomas A
Author_xml	– sequence: 1 givenname: Bérénice A surname: Benayoun fullname: Benayoun, Bérénice A organization: Department of Genetics, Stanford University, Stanford CA 94305, USA; Paul F. Glenn Laboratories for the Biology of Aging, Stanford University, Stanford CA 94305, USA – sequence: 2 givenname: Elizabeth A surname: Pollina fullname: Pollina, Elizabeth A organization: Department of Genetics, Stanford University, Stanford CA 94305, USA; Cancer Biology Program, Stanford University, Stanford CA 94305, USA – sequence: 3 givenname: Duygu surname: Ucar fullname: Ucar, Duygu organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 4 givenname: Salah surname: Mahmoudi fullname: Mahmoudi, Salah organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 5 givenname: Kalpana surname: Karra fullname: Karra, Kalpana organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 6 givenname: Edith D surname: Wong fullname: Wong, Edith D organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 7 givenname: Keerthana surname: Devarajan fullname: Devarajan, Keerthana organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 8 givenname: Aaron C surname: Daugherty fullname: Daugherty, Aaron C organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 9 givenname: Anshul B surname: Kundaje fullname: Kundaje, Anshul B organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 10 givenname: Elena surname: Mancini fullname: Mancini, Elena organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 11 givenname: Benjamin C surname: Hitz fullname: Hitz, Benjamin C organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 12 givenname: Rakhi surname: Gupta fullname: Gupta, Rakhi organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 13 givenname: Thomas A surname: Rando fullname: Rando, Thomas A organization: Paul F. Glenn Laboratories for the Biology of Aging, Stanford University, Stanford CA 94305, USA; Department of Neurology and Neurological Sciences, Stanford University, Stanford CA 94305, USA; RR&D REAP, VA Palo Alto Health Care Systems, Palo Alto, CA 94304,USA – sequence: 14 givenname: Julie C surname: Baker fullname: Baker, Julie C organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 15 givenname: Michael P surname: Snyder fullname: Snyder, Michael P organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 16 givenname: J Michael surname: Cherry fullname: Cherry, J Michael organization: Department of Genetics, Stanford University, Stanford CA 94305, USA – sequence: 17 givenname: Anne surname: Brunet fullname: Brunet, Anne email: anne.brunet@stanford.edu organization: Department of Genetics, Stanford University, Stanford CA 94305, USA; Paul F. Glenn Laboratories for the Biology of Aging, Stanford University, Stanford CA 94305, USA; Cancer Biology Program, Stanford University, Stanford CA 94305, USA. Electronic address: anne.brunet@stanford.edu
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25083876$$D View this record in MEDLINE/PubMed
BookMark	eNpNUDtPwzAYtFARfcAfYEAeWRL8-ZHHiCqgQCUWmCPH_iJcEifE7tB_TyqKxHSn0-l0d0sy871HQq6BpcAgu9ulBts25QxkyrKU8fyMLICVeSIh57N_fE6WIewYY4VS6oLMuWKFKPJsQV424lV2KGg9orbxk7pAW-e_0NLY02M-dRZ9dPFAtZ_EUftgRjdE13vdUtP74EJEbw6X5LzRbcCrE67Ix-PD-3qTbN-entf328QoJWMirCgbYxqlhESuOWCmJbNC2EwYrLUyDS8ASit5o5WF2uqyVFiDEdpCUfMVuf3NHcb-e48hVp0Lx6baY78PFSgF0xdFKSbrzcm6rzu01TC6To-H6m8__wE0LWAv
CitedBy_id	crossref_primary_10_1016_j_tma_2020_08_002 crossref_primary_10_1016_j_tcb_2017_10_008 crossref_primary_10_1038_srep37942 crossref_primary_10_3389_fnins_2015_00176 crossref_primary_10_3390_cells10061354 crossref_primary_10_3390_ijms252010979 crossref_primary_10_1007_s10620_019_05529_2 crossref_primary_10_1016_j_archoralbio_2023_105695 crossref_primary_10_1038_s41467_021_27719_1 crossref_primary_10_1016_j_molmed_2021_09_006 crossref_primary_10_3389_fphys_2021_686270 crossref_primary_10_1186_s41065_021_00182_0 crossref_primary_10_1038_s41421_020_00211_8 crossref_primary_10_1016_j_gene_2021_145862 crossref_primary_10_1186_s12864_024_10270_w crossref_primary_10_1038_s41420_023_01604_w crossref_primary_10_3390_ijms232112873 crossref_primary_10_1016_j_jbc_2025_108568 crossref_primary_10_1038_s41467_022_30484_4 crossref_primary_10_1007_s40495_016_0054_1 crossref_primary_10_1038_ng_3385 crossref_primary_10_1038_s41467_018_04426_y crossref_primary_10_3389_fcell_2023_1167111 crossref_primary_10_1038_s41392_023_01528_y crossref_primary_10_1073_pnas_1708044114 crossref_primary_10_3390_genes10050348 crossref_primary_10_1016_j_biotechadv_2021_107822 crossref_primary_10_3389_freae_2024_1451971 crossref_primary_10_1016_j_bbadis_2024_167226 crossref_primary_10_1093_bib_bbaa393 crossref_primary_10_1016_j_molcel_2014_10_008 crossref_primary_10_1073_pnas_2118938119 crossref_primary_10_3390_epigenomes3020011 crossref_primary_10_7554_eLife_17985 crossref_primary_10_1038_s41467_020_16539_4 crossref_primary_10_1186_s13059_018_1485_2 crossref_primary_10_1139_bcb_2019_0107 crossref_primary_10_1038_s41587_021_00869_9 crossref_primary_10_1002_advs_201801483 crossref_primary_10_1038_s41467_024_54740_x crossref_primary_10_1038_celldisc_2015_8 crossref_primary_10_1186_s13148_023_01556_z crossref_primary_10_1016_j_xgen_2024_100542 crossref_primary_10_1016_S0021_9258_17_49885_1 crossref_primary_10_1007_s12035_017_0427_4 crossref_primary_10_1002_bies_201500124 crossref_primary_10_1002_bies_202200239 crossref_primary_10_1038_nature19362 crossref_primary_10_1080_15592294_2016_1278095 crossref_primary_10_1093_procel_pwaf010 crossref_primary_10_1038_nsmb_2990 crossref_primary_10_1038_nature19360 crossref_primary_10_1186_s12864_019_5932_6 crossref_primary_10_2217_epi_2016_0062 crossref_primary_10_1371_journal_pone_0221270 crossref_primary_10_1007_s12035_016_0010_4 crossref_primary_10_1016_j_bbagrm_2020_194560 crossref_primary_10_1073_pnas_1414841112 crossref_primary_10_1093_nar_gkx251 crossref_primary_10_1016_j_gene_2023_147581 crossref_primary_10_3390_genes12081182 crossref_primary_10_1002_prot_25399 crossref_primary_10_1038_mt_2015_208 crossref_primary_10_1038_s41467_024_48990_y crossref_primary_10_1016_j_bbagrm_2020_194567 crossref_primary_10_1186_s13072_025_00601_w crossref_primary_10_1016_j_ccell_2018_10_014 crossref_primary_10_1101_gr_217075_116 crossref_primary_10_1007_s13277_015_4710_6 crossref_primary_10_1016_j_cell_2015_01_038 crossref_primary_10_15252_embr_202357020 crossref_primary_10_1111_nph_70349 crossref_primary_10_3390_biom11020143 crossref_primary_10_1093_nar_gkad105 crossref_primary_10_1016_j_csbj_2020_05_008 crossref_primary_10_1016_j_freeradbiomed_2021_03_043 crossref_primary_10_1111_febs_14728 crossref_primary_10_2337_db21_0706 crossref_primary_10_1016_j_immuni_2019_09_021 crossref_primary_10_1007_s11892_015_0635_0 crossref_primary_10_1101_gr_266924_120 crossref_primary_10_1080_10985549_2024_2388254 crossref_primary_10_1007_s00294_021_01196_x crossref_primary_10_1093_nar_gkac265 crossref_primary_10_1016_j_molmet_2025_102197 crossref_primary_10_3389_fgene_2021_701049 crossref_primary_10_1016_j_taap_2023_116747 crossref_primary_10_1038_s41467_024_51426_2 crossref_primary_10_1186_s12711_017_0331_4 crossref_primary_10_3390_epigenomes5020014 crossref_primary_10_1016_j_cell_2017_02_015 crossref_primary_10_1038_s41556_024_01433_8 crossref_primary_10_1038_s41598_022_25881_0 crossref_primary_10_1016_j_canlet_2024_216840 crossref_primary_10_1038_s41576_022_00468_7 crossref_primary_10_1038_s41525_021_00232_6 crossref_primary_10_1016_j_ygeno_2016_09_003 crossref_primary_10_1016_j_csbj_2022_07_004 crossref_primary_10_1186_s13075_016_1169_9 crossref_primary_10_3389_fcell_2025_1559183 crossref_primary_10_1093_nar_gkae498 crossref_primary_10_1074_jbc_RA119_011174 crossref_primary_10_3389_fgene_2023_1108104 crossref_primary_10_1242_dev_182568 crossref_primary_10_1002_jcp_30211 crossref_primary_10_1016_j_theriogenology_2020_02_036 crossref_primary_10_1155_2022_3490433 crossref_primary_10_1038_s41586_019_1658_5 crossref_primary_10_1038_s41598_023_39979_6 crossref_primary_10_15252_embr_202051264 crossref_primary_10_1002_ijc_35315 crossref_primary_10_1139_gen_2023_0011 crossref_primary_10_1146_annurev_pathmechdis_051122_110756 crossref_primary_10_1186_s12915_020_00842_z crossref_primary_10_1016_j_csbj_2021_12_027 crossref_primary_10_1101_gr_240093_118 crossref_primary_10_1007_s11357_023_00875_6 crossref_primary_10_1038_s41586_023_06585_5 crossref_primary_10_1016_j_ygeno_2015_07_003 crossref_primary_10_1038_s41467_021_24466_1 crossref_primary_10_1007_s00018_015_2125_6 crossref_primary_10_1073_pnas_1510510112 crossref_primary_10_7717_peerj_1750 crossref_primary_10_1002_bies_201600095 crossref_primary_10_1016_j_cels_2020_09_004 crossref_primary_10_1186_s13148_016_0179_4 crossref_primary_10_1242_dev_180018 crossref_primary_10_2217_epi_2018_0084 crossref_primary_10_1093_nar_gkae021 crossref_primary_10_3892_ijo_2018_4347 crossref_primary_10_1038_s41467_019_10502_8 crossref_primary_10_1371_journal_ppat_1008562 crossref_primary_10_3390_ani14091345 crossref_primary_10_1016_j_cbpa_2021_01_011 crossref_primary_10_1016_j_molcel_2016_10_019 crossref_primary_10_1093_hmg_ddv198 crossref_primary_10_3389_fonc_2022_916947 crossref_primary_10_1158_0008_5472_CAN_19_3228 crossref_primary_10_3390_cells10051209 crossref_primary_10_1016_j_nlm_2019_04_009 crossref_primary_10_2337_db20_1214 crossref_primary_10_1016_j_gene_2020_144758 crossref_primary_10_1016_j_cels_2016_03_009 crossref_primary_10_1016_j_diff_2016_03_001 crossref_primary_10_1128_MCB_00382_15 crossref_primary_10_1093_bioadv_vbae074 crossref_primary_10_1101_gad_349803_122 crossref_primary_10_1186_s13059_025_03618_2 crossref_primary_10_1126_sciadv_adu5653 crossref_primary_10_1016_j_omto_2020_05_004 crossref_primary_10_1016_j_cels_2020_11_001 crossref_primary_10_1038_leu_2016_182 crossref_primary_10_1038_cdd_2017_157 crossref_primary_10_1016_j_asjsur_2025_05_096 crossref_primary_10_1186_s40478_021_01234_2 crossref_primary_10_1038_s41597_024_03648_8 crossref_primary_10_3389_fpls_2021_761059 crossref_primary_10_1186_s12935_022_02552_8 crossref_primary_10_1016_j_ceb_2016_12_009 crossref_primary_10_1038_s41586_025_09400_5 crossref_primary_10_1038_s41598_023_38879_z crossref_primary_10_1016_j_semcancer_2018_11_001 crossref_primary_10_1073_pnas_2406519121 crossref_primary_10_1016_j_cmet_2023_02_001 crossref_primary_10_1146_annurev_genom_083117_021632 crossref_primary_10_15252_embj_201796764 crossref_primary_10_1016_j_neuint_2024_105853 crossref_primary_10_1038_s41467_023_40776_y crossref_primary_10_1007_s12031_017_0901_5 crossref_primary_10_1080_15592294_2016_1265713 crossref_primary_10_1080_13543784_2022_2158810 crossref_primary_10_1038_s41388_022_02311_z crossref_primary_10_1534_genetics_118_301525 crossref_primary_10_3390_biomedicines10040748 crossref_primary_10_3390_cells11182830 crossref_primary_10_7554_eLife_103417_3 crossref_primary_10_1002_stem_2711 crossref_primary_10_1080_15592294_2018_1514231 crossref_primary_10_1038_s41598_020_65006_z crossref_primary_10_1038_s41467_020_18303_0 crossref_primary_10_1016_j_stem_2014_10_019 crossref_primary_10_1210_endocr_bqab128 crossref_primary_10_1016_j_molcel_2017_02_005 crossref_primary_10_1038_s41594_020_0443_3 crossref_primary_10_1101_gr_276126_121 crossref_primary_10_1161_ATVBAHA_115_305561 crossref_primary_10_1007_s40610_016_0031_9 crossref_primary_10_1038_cdd_2017_32 crossref_primary_10_1038_ncb3629 crossref_primary_10_1038_s41467_018_04552_7 crossref_primary_10_1128_MCB_00677_15 crossref_primary_10_1016_j_cmet_2021_06_005 crossref_primary_10_2217_epi_2019_0290 crossref_primary_10_1007_s11060_018_03029_3 crossref_primary_10_1038_s41388_025_03314_2 crossref_primary_10_1038_s41598_024_55189_0 crossref_primary_10_1016_j_stem_2016_06_020 crossref_primary_10_1371_journal_pcbi_1005585 crossref_primary_10_1084_jem_20160185 crossref_primary_10_1371_journal_pone_0225801 crossref_primary_10_1016_j_dib_2015_08_037 crossref_primary_10_1016_j_molcel_2017_10_013 crossref_primary_10_1186_s13072_018_0212_2 crossref_primary_10_1016_j_molcel_2018_04_028 crossref_primary_10_1007_s11427_016_5028_3 crossref_primary_10_1111_cas_15623 crossref_primary_10_3390_vaccines13070768 crossref_primary_10_1016_j_gpb_2016_09_001 crossref_primary_10_3390_epigenomes3030013 crossref_primary_10_1101_gad_350594_123 crossref_primary_10_1186_s13059_017_1159_5 crossref_primary_10_1186_s13062_016_0148_z crossref_primary_10_1007_s00335_016_9645_8 crossref_primary_10_1186_s13040_015_0051_7 crossref_primary_10_26508_lsa_202101302 crossref_primary_10_5498_wjp_v11_i10_711 crossref_primary_10_1161_CIRCULATIONAHA_116_026991 crossref_primary_10_1101_gr_240606_118 crossref_primary_10_1186_s12864_024_10068_w crossref_primary_10_1007_s00439_023_02529_1 crossref_primary_10_1158_0008_5472_CAN_17_3498 crossref_primary_10_1016_j_cancergen_2015_02_008 crossref_primary_10_1016_j_stem_2015_08_009 crossref_primary_10_1016_j_exer_2021_108663 crossref_primary_10_1007_s12035_019_1486_5 crossref_primary_10_1016_j_redox_2025_103598 crossref_primary_10_1159_000514305 crossref_primary_10_3389_fgene_2021_749850 crossref_primary_10_1016_j_cels_2017_10_003 crossref_primary_10_1111_nyas_12956 crossref_primary_10_1038_s41467_021_22024_3 crossref_primary_10_1038_s41598_017_02257_3 crossref_primary_10_1016_j_tcb_2019_03_003 crossref_primary_10_1038_s41598_017_14389_7 crossref_primary_10_1128_microbiolspec_MCHD_0010_2015 crossref_primary_10_1038_nrm4048 crossref_primary_10_1042_BJ20140496 crossref_primary_10_1038_s41375_025_02638_y crossref_primary_10_1038_s41598_021_98889_7 crossref_primary_10_1016_j_molcel_2018_08_015 crossref_primary_10_1016_j_tig_2021_06_017 crossref_primary_10_1038_tp_2015_169 crossref_primary_10_1016_j_isci_2022_104235 crossref_primary_10_1371_journal_pone_0144398 crossref_primary_10_1038_s41467_021_21707_1 crossref_primary_10_1016_j_bbagrm_2024_195009 crossref_primary_10_1016_j_gpb_2020_10_008 crossref_primary_10_1371_journal_pone_0151917 crossref_primary_10_1007_s12185_016_2118_8 crossref_primary_10_1093_nar_gkad198 crossref_primary_10_2217_epi_14_87 crossref_primary_10_1007_s00125_023_05896_6 crossref_primary_10_1155_2016_7276161 crossref_primary_10_1038_s41580_021_00335_z crossref_primary_10_1038_s44318_024_00329_5 crossref_primary_10_1016_j_celrep_2025_115619 crossref_primary_10_1038_ncomms15011 crossref_primary_10_1186_s13072_018_0251_8 crossref_primary_10_1136_jitc_2022_005693 crossref_primary_10_1016_j_bpj_2019_04_006 crossref_primary_10_1016_j_exppara_2018_07_001 crossref_primary_10_3389_fmolb_2023_1273046 crossref_primary_10_1038_s41467_024_50725_y crossref_primary_10_1186_s12885_018_4580_6 crossref_primary_10_1016_j_mce_2016_07_010 crossref_primary_10_1016_j_scib_2017_09_011 crossref_primary_10_1093_molehr_gaad019 crossref_primary_10_1038_s41588_018_0234_5 crossref_primary_10_1063_5_0271050 crossref_primary_10_7554_eLife_104076_3 crossref_primary_10_1016_j_tcb_2024_06_001 crossref_primary_10_1038_ng_3740 crossref_primary_10_1016_j_cmet_2017_07_019 crossref_primary_10_1016_j_tig_2019_12_004 crossref_primary_10_1038_s41467_022_32165_8 crossref_primary_10_1186_s12915_024_01903_3 crossref_primary_10_1371_journal_pcbi_1008926 crossref_primary_10_1016_j_compbiolchem_2016_07_006 crossref_primary_10_1016_j_jplph_2020_153167 crossref_primary_10_1186_s13059_023_02977_y crossref_primary_10_15252_embj_201696035 crossref_primary_10_1371_journal_pgen_1007466 crossref_primary_10_1016_j_devcel_2017_01_013 crossref_primary_10_1093_nar_gkv1354 crossref_primary_10_1074_jbc_M115_672931 crossref_primary_10_1038_s41467_018_03904_7 crossref_primary_10_3390_cancers13051025 crossref_primary_10_1139_bcb_2023_0211 crossref_primary_10_1007_s00018_019_03143_z crossref_primary_10_1093_nar_gkaf632 crossref_primary_10_1016_j_molcel_2016_05_013 crossref_primary_10_1007_s40484_020_0225_2 crossref_primary_10_1016_j_cmet_2018_04_013 crossref_primary_10_1101_gr_225896_117 crossref_primary_10_1186_s12864_019_5729_7 crossref_primary_10_1158_1541_7786_MCR_23_0454 crossref_primary_10_1016_j_molcel_2015_04_022 crossref_primary_10_1038_s41598_017_03665_1 crossref_primary_10_1111_jcmm_13238 crossref_primary_10_1186_s13148_021_01126_1 crossref_primary_10_1016_j_cmet_2015_08_024 crossref_primary_10_1104_pp_114_252999 crossref_primary_10_1038_s41598_017_12335_1 crossref_primary_10_1139_gen_2020_0124 crossref_primary_10_1038_s41467_023_38002_w crossref_primary_10_1186_s11658_025_00690_1 crossref_primary_10_1186_s13072_016_0110_4 crossref_primary_10_1038_ng_3402 crossref_primary_10_1093_nar_gkaf720 crossref_primary_10_1093_nar_gkad300 crossref_primary_10_7554_eLife_104076 crossref_primary_10_1101_gr_183368_114 crossref_primary_10_1515_hsz_2017_0190 crossref_primary_10_1016_j_envpol_2024_123396 crossref_primary_10_3390_plants13152079 crossref_primary_10_3390_ijms20092158 crossref_primary_10_1038_s41422_025_01080_0 crossref_primary_10_1016_j_tcb_2018_09_002 crossref_primary_10_1158_0008_5472_CAN_18_3663 crossref_primary_10_1016_j_stem_2017_03_003 crossref_primary_10_1038_ni_3818 crossref_primary_10_3390_ijms252413545 crossref_primary_10_7554_eLife_13087 crossref_primary_10_7554_eLife_103417 crossref_primary_10_1186_s13072_024_00542_w crossref_primary_10_4049_jimmunol_1400789 crossref_primary_10_1111_febs_15219 crossref_primary_10_1016_j_chemosphere_2023_138900 crossref_primary_10_1097_MOH_0000000000000346 crossref_primary_10_15252_msb_20199156 crossref_primary_10_1038_s41467_022_35059_x crossref_primary_10_1002_bies_201700124 crossref_primary_10_1007_s42994_025_00223_6 crossref_primary_10_1038_ncomms12331 crossref_primary_10_1038_s41467_022_33433_3 crossref_primary_10_1002_1873_3468_12424
ContentType	Journal Article
Copyright	Copyright © 2014 Elsevier Inc. All rights reserved.
Copyright_xml	– notice: Copyright © 2014 Elsevier Inc. All rights reserved.
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1016/j.cell.2014.06.027
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Biology
EISSN	1097-4172
ExternalDocumentID	25083876
Genre	Research Support, U.S. Gov't, Non-P.H.S Meta-Analysis Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural
GrantInformation_xml	– fundername: NIA NIH HHS grantid: P01 AG036695 – fundername: NIA NIH HHS grantid: F31 AG043232 – fundername: NIGMS NIH HHS grantid: P01 GM099130 – fundername: NCI NIH HHS grantid: T32 CA009302 – fundername: NIA NIH HHS grantid: DP1 AG044848 – fundername: NIGMS NIH HHS grantid: T32 GM007790 – fundername: NIGMS NIH HHS grantid: R01 GM103787
GroupedDBID	--- --K -DZ -ET -~X 0R~ 0WA 1RT 1~5 29B 2FS 2WC 3EH 4.4 457 4G. 53G 5GY 5RE 5VS 62- 6J9 7-5 85S AAEDT AAEDW AAFWJ AAHBH AAKRW AAKUH AALRI AAMRU AAQFI AAVLU AAXUO AAYJJ AAYWO ABCQX ABDGV ABJNI ABMAC ABOCM ACGFO ACGFS ACNCT ACVFH ADBBV ADCNI ADEZE ADVLN ADXHL AEFWE AENEX AEUPX AEXQZ AFPUW AFTJW AGCQF AGHFR AGHSJ AGKMS AHHHB AIDAL AIGII AITUG AKAPO AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ APXCP ASPBG AVWKF AZFZN BAWUL CGR CS3 CUY CVF DIK DU5 E3Z EBS ECM EFKBS EIF EJD F5P FCP FDB FIRID HH5 IH2 IHE IXB J1W JIG K-O KOO KQ8 L7B LX5 M3Z M41 N9A NPM O-L O9- OK1 P2P RIG RNS ROL RPZ SCP SDG SDP SES SSZ TAE TN5 TR2 TWZ UKR UPT WH7 YYQ YZZ ZCA 7X8
ID	FETCH-LOGICAL-c554t-3d39fccf5534e2a21e6a40d33d63ceba5cf28119d42fa5d1bda995eb1c3ad18b2
IEDL.DBID	7X8
ISICitedReferencesCount	384
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000340944300018&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1097-4172
IngestDate	Sat Sep 27 21:56:05 EDT 2025 Mon Jul 21 06:05:59 EDT 2025
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	3
Language	English
License	Copyright © 2014 Elsevier Inc. All rights reserved.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c554t-3d39fccf5534e2a21e6a40d33d63ceba5cf28119d42fa5d1bda995eb1c3ad18b2
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	https://dx.doi.org/10.1016/j.cell.2014.06.027
PMID	25083876
PQID	1551027893
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_1551027893 pubmed_primary_25083876
PublicationCentury	2000
PublicationDate	2014-07-31
PublicationDateYYYYMMDD	2014-07-31
PublicationDate_xml	– month: 07 year: 2014 text: 2014-07-31 day: 31
PublicationDecade	2010
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Cell
PublicationTitleAlternate	Cell
PublicationYear	2014
References	Cell. 2015 Nov 19;163(5):1281-6
References_xml	– reference: - Cell. 2015 Nov 19;163(5):1281-6
SSID	ssj0008555
Score	2.6079977
SecondaryResourceType	review_article
Snippet	Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to mark the transcription start sites of active genes. Here, we show that...
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	673
SubjectTerms	Animals Artificial Intelligence Cells - metabolism Genomics Histone Code Histones - metabolism Humans Lysine - metabolism Methylation Mice, Inbred C57BL Neural Stem Cells - metabolism RNA Polymerase II - metabolism Transcription, Genetic
Title	H3K4me3 breadth is linked to cell identity and transcriptional consistency
URI	https://www.ncbi.nlm.nih.gov/pubmed/25083876 https://www.proquest.com/docview/1551027893
Volume	158
WOSCitedRecordID	wos000340944300018&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8NAEF7UKnjx_agvVvC62H0l2ZOIWIqP0oNKb2GzD-yhSTVR6L93J0npSRC85BBIGDazM19mvp0PoSudyKQnnSSRMhERinuiNWXEWWd1bOOeqVmVb0_xcJiMx2rUFtzKlla5iIl1oLaFgRr5NaR26JIpfjP7IKAaBd3VVkJjFXV4gDJA6YrHy2nhiaxVT6HJSkTI1O2hmYbfBYVxoHaJen4ni3-HmHWq6W__18gdtNWCTHzbeMUuWnH5HtpoZCfn--hhwB_F1HEcfoe1rd7xpMTQyHUWVwUGi_GkOb87xzoPNyGfLaJLeK8BUm0JYHt-gF779y93A9JqKhATgENFuOXKG-Ol5MIxzaiLtOhZzm3Ejcu0NJ4llCormNfS0sxqpWQI6IZrS5OMHaK1vMjdMcJUm8w475mGMqIHBQ8qnFTKZj7gAtdFl4tFSoPPgvU6d8VXmS6XqYuOmpVOZ81wjZTBfPoQok_-8PQp2oQP2JRaz1DHhx3rztG6-a4m5edF7QzhOhw9_wBWu7-W
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=H3K4me3+breadth+is+linked+to+cell+identity+and+transcriptional+consistency&rft.jtitle=Cell&rft.au=Benayoun%2C+B%C3%A9r%C3%A9nice+A&rft.au=Pollina%2C+Elizabeth+A&rft.au=Ucar%2C+Duygu&rft.au=Mahmoudi%2C+Salah&rft.date=2014-07-31&rft.issn=1097-4172&rft.eissn=1097-4172&rft.volume=158&rft.issue=3&rft.spage=673&rft_id=info:doi/10.1016%2Fj.cell.2014.06.027&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1097-4172&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1097-4172&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1097-4172&client=summon