Evaluation of viral concentration methods from irrigation and processing water

► We compared four viral concentration methods for their recovery in water. ► MNV-1 and MS2 phages were used as surrogate viruses during this study. ► All methods were evaluated in processing water and four types of irrigation water. ► The best method used a negatively charged membrane with the Tr a...

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Bibliographic Details
Published in:Journal of virological methods Vol. 187; no. 2; pp. 294 - 303
Main Authors: De Keuckelaere, Ann, Baert, Leen, Duarte, Alexandra, Stals, Ambroos, Uyttendaele, Mieke
Format: Journal Article
Language:English
Published: Kidlington Elsevier B.V 01.02.2013
Elsevier
Subjects:
Adsorption
bacteriophages
Biological and medical sciences
filtration
Filtration - methods
Fundamental and applied biological sciences. Psychology
Hepatitis A virus
Hepatitis A virus - isolation & purification
Hepatovirus A
humans
irrigation
irrigation water
Levivirus - isolation & purification
mice
Microbiology
Murine norovirus
Norovirus
Norovirus - isolation & purification
pathogens
rain
Real-Time Polymerase Chain Reaction
Real-time RT-PCR
Reverse Transcriptase Polymerase Chain Reaction
river water
Rotavirus
Rotavirus - isolation & purification
Specimen Handling - methods
Techniques used in virology
Viral Plaque Assay
Virology
Virology - methods
Water
Water Microbiology
Real-time RT-PCR
Water
Hepatitis A virus
Norovirus
Rotavirus
Murine norovirus
Picornaviridae
Rodentia
Method
Irrigation water
Real time
Reoviridae
Hepatovirus
Virus
Vertebrata
Mammalia
Mouse
Caliciviridae
Reverse transcription polymerase chain reaction
ISSN:0166-0934, 1879-0984, 1879-0984
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Summary:► We compared four viral concentration methods for their recovery in water. ► MNV-1 and MS2 phages were used as surrogate viruses during this study. ► All methods were evaluated in processing water and four types of irrigation water. ► The best method used a negatively charged membrane with the Tr alk elution buffer. ► The best method was tested for its recovery of HAV, GI and GII NoV and RV. Four viral concentration methods were evaluated for their efficiency in recovering murine norovirus-1 (MNV-1) (surrogate for human noroviruses (NoV)) and MS2 bacteriophages from processing water (1L) and four different types of irrigation water (bore hole water, rain water, open well and river water) (2–5L). Three methods were based on the viral adsorption and elution principle, two methods using an electronegative HA-membrane (Katayama et al., 2002), one method using an electropositive Zetapor membrane according to CEN/TC275/WG6/TAG4 and the fourth method was based on size exclusion using a tangential flow filtration system. Detection of MNV-1 was achieved by real-time RT-PCR and detection of MS2 by double-layer plaque assay. For the recovery of MNV-1, the method using an electronegative HA-filter in combination with an elution buffer earlier optimized by Hamza et al. (2009) (Method 1) performed best for all types of water (recovery: 5.8–21.9%). In case of MS2 detection, the best method depended upon the type of water although Method 1 provided the most consistent recovery. To complete this evaluation, the Method 1 was evaluated further for the concentration of human enteric viruses (GI and GII NoV, hepatitis A virus (HAV) and rotaviruses) in the same five types of water. Although detection of rotaviruses (RV) was somewhat less efficient, Method 1 proved reliable for the detection of NoV and HAV in all water types. Mean recovery efficiencies ranging from 4.8% for detection of GI NoV in open well water to 32.1% for detection of HAV in bore hole water, depending on the water type and the viral pathogen analyzed.
Bibliography:http://dx.doi.org/10.1016/j.jviromet.2012.11.028
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ISSN:0166-0934
1879-0984
1879-0984
DOI:10.1016/j.jviromet.2012.11.028