Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast

The understanding of the molecular mechanisms underlying acquired herbicide resistance is crucial in dealing with the emergence of resistant weeds. Saccharomyces cerevisiae has been used as a model system to gain insights into the mechanisms underlying resistance to the herbicide 2,4-dichlorophenoxy...

Full description

Saved in:

Bibliographic Details
Published in:	Applied microbiology and biotechnology Vol. 84; no. 5; pp. 927 - 936
Main Authors:	Cabrito, Tânia R, Teixeira, Miguel C, Duarte, Alexandra A, Duque, Paula, Sá-Correia, Isabel
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2009 Springer Berlin Heidelberg Springer Nature B.V
Subjects:	2,4-D 2,4-Dichlorophenoxyacetic Acid 2,4-Dichlorophenoxyacetic Acid - metabolism 2,4-Dichlorophenoxyacetic Acid - pharmacology Acetic acid Acid resistance aluminum Antiporters Antiporters - genetics Antiporters - metabolism Applied Genetics and Molecular Biotechnology Arabidopsis Proteins Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biotechnology Cell division Dichlorophenoxyacetic acid drug effects Gene Expression Genes genetics Herbicide Resistance Herbicides Herbicides - metabolism Herbicides - pharmacology indole acetic acid Indoleacetic acid Intracellular Life Sciences Localization Membrane Transport Proteins Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Membranes metabolism Microbial Genetics and Genomics Microbiology Molecular biology Molecular modelling Multidrug resistance multiple drug resistance open reading frames Organic Cation Transport Proteins Organic Cation Transport Proteins - genetics Organic Cation Transport Proteins - metabolism Organic Chemicals Organic Chemicals - metabolism Organic Chemicals - pharmacology pharmacology Physiology Plant resistance Plasma plasma membrane Plasmids Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Studies Thallium Toxicity transporters Weeds Yeast Yeasts Plant MFS transporter Herbicide resistance 2,4-D IAA and metal resistance
ISSN:	0175-7598, 1432-0614
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The understanding of the molecular mechanisms underlying acquired herbicide resistance is crucial in dealing with the emergence of resistant weeds. Saccharomyces cerevisiae has been used as a model system to gain insights into the mechanisms underlying resistance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The TPO1 gene, encoding a multidrug resistance (MDR) plasma membrane transporter of the major facilitator superfamily (MFS), was previously found to confer resistance to 2,4-D in yeast and to be transcriptionally activated in response to the herbicide. In this work, we demonstrate that Tpo1p is required to reduce the intracellular concentration of 2,4-D. ScTpo1p homologs encoding putative plasma membrane MFS transporters from the plant model Arabidopsis thaliana were analyzed for a possible role in 2,4-D resistance. At5g13750 was chosen for further analysis, as its transcript levels were found to increase in 2,4-D stressed plants. The functional heterologous expression of this plant open reading frame in yeast was found to confer increased resistance to the herbicide in Δtpo1 and wild-type cells, through the reduction of the intracellular concentration of 2,4-D. Heterologous expression of At5g13750 in yeast also leads to increased resistance to indole-3-acetic acid (IAA), Al³⁺ and Tl³⁺. At5g13750 is the first plant putative MFS transporter to be suggested as possibly involved in MDR.
Bibliography:	http://dx.doi.org/10.1007/s00253-009-2025-5 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0175-7598 1432-0614
DOI:	10.1007/s00253-009-2025-5