Microbial signature profiles of periodontally healthy and diseased patients

Aim To determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases. Methods Subgingival biofilm was obtained from patients with periodontal health (27), gingivitis (11), chronic periodontitis (35) and aggressive periodontitis (24), and analysed for...

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Published in:Journal of clinical periodontology Vol. 41; no. 11; pp. 1027 - 1036
Main Authors: Lourenço, Talita Gomes Baêta, Heller, Débora, Silva-Boghossian, Carina Maciel, Cotton, Sean L., Paster, Bruce J., Colombo, Ana Paula Vieira
Format: Journal Article
Language:English
Published: United States Blackwell Publishing Ltd 01.11.2014
Subjects:
Adult
Aggregatibacter actinomycetemcomitans - isolation & purification
aggressive periodontitis
Aggressive Periodontitis - microbiology
Bacteria - classification
Bacteria - isolation & purification
Bacteroides - isolation & purification
Bacteroides forsythus
biofilm
Biofilms
Cardiobacterium - classification
Carnobacteriaceae - isolation & purification
chronic periodontitis
Chronic Periodontitis - microbiology
Dentistry
Female
Fusobacterium
Fusobacterium - classification
Fusobacterium nucleatum
Fusobacterium nucleatum - isolation & purification
gingivitis
Gingivitis - microbiology
Granulicatella adiacens
Granulicatella elegans
Gum disease
Humans
Male
microarray analysis
Microbiology
Microbiota
Neisseria - classification
Neisseria polysaccharea
Peptostreptococcus - classification
Periodontal Attachment Loss - microbiology
Periodontal Index
Periodontal Pocket - microbiology
Periodontium - microbiology
Porphyromonas
Porphyromonas - classification
Porphyromonas - isolation & purification
Porphyromonas endodontalis
Porphyromonas endodontalis - isolation & purification
Prevotella - classification
Prevotella denticola
Young Adult
microbiota
aggressive periodontitis
gingivitis
microarray analysis
chronic periodontitis
biofilm
ISSN:0303-6979, 1600-051X, 1600-051X
Online Access:Get full text
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Summary:Aim To determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases. Methods Subgingival biofilm was obtained from patients with periodontal health (27), gingivitis (11), chronic periodontitis (35) and aggressive periodontitis (24), and analysed for the presence of >250 species/phylotypes using HOMIM. Microbial differences among groups were examined by Mann–Whitney U‐test. Regression analyses were performed to determine microbial risk indicators of disease. Results Putative and potential new periodontal pathogens were more prevalent in subjects with periodontal diseases than periodontal health. Detection of Porphyromonas endodontalis/Porphyromonas spp. (OR 9.5 [1.2–73.1]) and Tannerella forsythia (OR 38.2 [3.2–450.6]), and absence of Neisseria polysaccharea (OR 0.004 [0–0.15]) and Prevotella denticola (OR 0.014 [0–0.49], p < 0.05) were risk indicators of periodontal disease. Presence of Aggregatibacter actinomycetemcomitans (OR 29.4 [3.4–176.5]), Cardiobacterium hominis (OR 14.9 [2.3–98.7]), Peptostreptococcaceae sp. (OR 35.9 [2.7–483.9]), P. alactolyticus (OR 31.3 [2.1–477.2]), and absence of Fretibacterium spp. (OR 0.024 [0.002–0.357]), Fusobacterium naviforme/Fusobacterium nucleatum ss vincentii (OR 0.015 [0.001–0.223]), Granulicatella adiacens/Granulicatella elegans (OR 0.013 [0.001–0.233], p < 0.05) were associated with aggressive periodontitis. Conclusion There were specific microbial signatures of the subgingival biofilm that were able to distinguish between microbiomes of periodontal health and diseases. Such profiles may be used to establish risk of disease. View the pubcast on this paper at http://www.scivee.tv/journalnode/63057
Bibliography:istex:DCBCEF0B3EC367CC13D4FCA46B2B37B2A5311851
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Figure S1. Frequency of scores (0 a 5) of the species/phylotypes detected by HOMIM in subgingival plaque samples of patients with periodontal health (n = 27) and gingivitis (n = 11).Figure S2. Frequency of scores (0 a 5) of the species/phylotypes detected by HOMIM in subgingival plaque samples of patients with gingivitis (n = 11) and periodontitis (n = 59).Figure S3. Frequency of scores (0 a 5) of the species/phylotypes detected by HOMIM in subgingival plaque samples of patients with chronic periodontitis (n = 35) and aggressive periodontitis (n = 24).Table S1. Demographic and clinical features of the study population distributed in the clinical groups.
ArticleID:JCPE12302
The National Institutes of Health - No. DE021565
Foundation for Research
State of Rio de Janeiro (FAPERJ)
Coordination of Improvement of Higher Education Personnel (CAPES)
National Council for Scientific and Technological Development (CNPq)
Conflict of interest and source of funding statement
The authors declare that they have no conflict of interests regarding this study. This work was supported in part by National Council for Scientific and Technological Development CNPq, and Coordination of Improvement of Higher Education Personnel (CAPES), Brasilia, Brazil; Foundation for Research Financial Support in the State of Rio de Janeiro (FAPERJ), Rio de Janeiro, Brazil and a grant from The National Institutes of Health, DE021565 (BP).
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ISSN:0303-6979
1600-051X
1600-051X
DOI:10.1111/jcpe.12302