The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications

Transflection-mode FTIR spectroscopy has become a popular method of measuring spectra from biomedical and other samples due to the relative low cost of substrates compared to transmission windows, and a higher absorbance due to a double pass through the same sample approximately doubling the effecti...

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Veröffentlicht in:Analyst (London) Jg. 138; H. 1; S. 144
Hauptverfasser: Bassan, Paul, Lee, Joe, Sachdeva, Ashwin, Pissardini, Juliana, Dorling, Konrad M, Fletcher, John S, Henderson, Alex, Gardner, Peter
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 01.01.2013
Schlagworte:
Absorption
Cytosine - chemistry
Humans
Male
Microscopy
Prostate - cytology
Prostate - pathology
Spectroscopy, Fourier Transform Infrared
ISSN:1364-5528, 1364-5528
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Zusammenfassung:Transflection-mode FTIR spectroscopy has become a popular method of measuring spectra from biomedical and other samples due to the relative low cost of substrates compared to transmission windows, and a higher absorbance due to a double pass through the same sample approximately doubling the effective path length. In this publication we state an optical description of samples on multilayer low-e reflective substrates. Using this model we are able to explain in detail the so-called electric-field standing wave effect and rationalise the non-linear change in absorbance with sample thickness. The ramifications of this non-linear change, for imaging and classification systems, where a model is built from tissue sectioned at a particular thickness and compared with tissue of a different thickness are discussed. We show that spectra can be distorted such that classification fails leading to inaccurate tissue segmentation which may have subsequent implications for disease diagnostics applications.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1364-5528
1364-5528
DOI:10.1039/c2an36090j