Mouse multipotent progenitor 5 cells are located at the interphase between hematopoietic stem and progenitor cells

Hematopoietic stem cells (HSCs) and distinct multipotent progenitor populations (MPP1-4) contained within the Lin- Sca-1+ c-Kit+ (LSK) compartment have previously been identified using diverse surface marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+ CD135-...

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Vydané v:Blood Ročník 137; číslo 23; s. 3218
Hlavní autori: Sommerkamp, Pia, Romero-Mulero, Mari Carmen, Narr, Andreas, Ladel, Luisa, Hustin, Lucie Sylvie Pierrette, Schönberger, Katharina, Renders, Simon, Altamura, Sandro, Zeisberger, Petra, Jäcklein, Karin, Klimmeck, Daniel, Rodriguez-Fraticelli, Alejo Ezequiel, Camargo, Fernando, Perié, Leila, Trumpp, Andreas, Cabezas-Wallscheid, Nina
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 10.06.2021
ISSN:1528-0020, 1528-0020
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Shrnutí:Hematopoietic stem cells (HSCs) and distinct multipotent progenitor populations (MPP1-4) contained within the Lin- Sca-1+ c-Kit+ (LSK) compartment have previously been identified using diverse surface marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+ CD135- CD48- CD150-). Upon transplantation, MPP5 support initial emergency myelopoiesis followed by stable contribution to the lymphoid lineage. Since MPP5 are capable of generating MPP1-4, but not HSCs, they represent a dynamic and versatile component of the MPP network. To characterize all hematopoietic stem and progenitor cells (HSPCs), we performed RNA-seq analysis to identify specific transcriptomic landscapes of HSCs and MPP1-5. This was complemented by single-cell (sc) RNA-seq analysis of LSK cells to establish the differentiation trajectories from HSCs to MPP1-5. In agreement with the functional reconstitution activity, MPP5 are located immediately downstream of HSCs but upstream of the more committed MPP2-4. This study provides a comprehensive analysis of the LSK compartment, focusing on the functional and molecular characteristics of the newly defined MPP5 subset.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1528-0020
1528-0020
DOI:10.1182/blood.2020007876