Development of a luminex-based assay for the detection of anti-capripoxvirus and rift valley fever virus antibodies in domestic ruminants

The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we...

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Vydáno v:	Virology journal Ročník 21; číslo 1; s. 335
Hlavní autoři:	Berguido, Francisco J., Settypalli, Tirumala Bharani Kumar, Mbuyi, Curé Georges Tshilenge, Bakhom, Mame Thierno, van Vuren, Petrus Jansen, Pawęska, Janusz T., Cattoli, Giovanni, Grabherr, Reingard, Lamien, Charles Euloge
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London BioMed Central 27.12.2024 BioMed Central Ltd BMC
Témata:	Animals Antibodies Antibodies, Viral - blood Antibodies, Viral - immunology Biological assay Biomedical and Life Sciences Biomedicine Bunyaviruses Capripoxvirus Capripoxvirus - genetics Capripoxvirus - immunology Cattle Cattle Diseases - diagnosis Cattle Diseases - immunology Cattle Diseases - virology cost effectiveness disease surveillance DNA viruses genus geographical distribution Goat Diseases - diagnosis Goat Diseases - immunology Goat Diseases - virology Goatpox virus Goats GTPV Health aspects immunologic techniques LSDV Luminex Lumpy skin disease virus Physiological aspects Poxviridae Infections - diagnosis Poxviridae Infections - immunology Poxviridae Infections - veterinary Poxviridae Infections - virology Rift Valley Fever - diagnosis Rift Valley Fever - immunology Rift Valley fever phlebovirus Rift Valley fever virus - immunology Ruminants Ruminants - immunology Ruminants - virology Sensitivity and Specificity Serologic Tests - methods Serologic Tests - veterinary Serology Sheep Sheep Diseases - diagnosis Sheep Diseases - immunology Sheep Diseases - virology Sheeppox virus species SPPV Testing vaccination Viral antibodies Virology Austria Rift valley fever GTPV A34 RVFV SPPV Capripoxvirus LSDV Serology Luminex
ISSN:	1743-422X, 1743-422X
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Shrnutí:	The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we developed and evaluated a Luminex assay for the simultaneous detection of antibodies against CaPV and RVFV in domestic ruminants. In cattle, the assay had a sensitivity (Se) of 98.7% and a specificity (Sp) of 98.3% in detecting anti-LSDV antibodies; both diagnostic parameters were 100% for the detection of anti-RVFV antibodies in this species. In sheep and goats, Se and Sp were 100% for the detection of anti-SPPV and anti-GTPV antibodies while they were 100% and 98.9%, respectively for the detection of anti-RVFV antibody. The assay did not cross react with anti-parapoxvirus antibodies of cattle, sheep, and goats. This multiplex serological assay offers a practical tool for accurate detection and monitoring of the immunological status of domestic ruminant populations against veterinary and socio-economically important capripox- and phleboviral infections, thus has the potential to aid in the strategic application of vaccination programmes.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3
ISSN:	1743-422X 1743-422X
DOI:	10.1186/s12985-024-02602-9