Development of a luminex-based assay for the detection of anti-capripoxvirus and rift valley fever virus antibodies in domestic ruminants

The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we...

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Veröffentlicht in:Virology journal Jg. 21; H. 1; S. 335
Hauptverfasser: Berguido, Francisco J., Settypalli, Tirumala Bharani Kumar, Mbuyi, Curé Georges Tshilenge, Bakhom, Mame Thierno, van Vuren, Petrus Jansen, Pawęska, Janusz T., Cattoli, Giovanni, Grabherr, Reingard, Lamien, Charles Euloge
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London BioMed Central 27.12.2024
BioMed Central Ltd
BMC
Schlagworte:
Animals
Antibodies
Antibodies, Viral - blood
Antibodies, Viral - immunology
Biological assay
Biomedical and Life Sciences
Biomedicine
Bunyaviruses
Capripoxvirus
Capripoxvirus - genetics
Capripoxvirus - immunology
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - immunology
Cattle Diseases - virology
cost effectiveness
disease surveillance
DNA viruses
genus
geographical distribution
Goat Diseases - diagnosis
Goat Diseases - immunology
Goat Diseases - virology
Goatpox virus
Goats
GTPV
Health aspects
immunologic techniques
LSDV
Luminex
Lumpy skin disease virus
Physiological aspects
Poxviridae Infections - diagnosis
Poxviridae Infections - immunology
Poxviridae Infections - veterinary
Poxviridae Infections - virology
Rift Valley Fever - diagnosis
Rift Valley Fever - immunology
Rift Valley fever phlebovirus
Rift Valley fever virus - immunology
Ruminants
Ruminants - immunology
Ruminants - virology
Sensitivity and Specificity
Serologic Tests - methods
Serologic Tests - veterinary
Serology
Sheep
Sheep Diseases - diagnosis
Sheep Diseases - immunology
Sheep Diseases - virology
Sheeppox virus
species
SPPV
Testing
vaccination
Viral antibodies
Virology
Austria
Rift valley fever
GTPV
A34
RVFV
SPPV
Capripoxvirus
LSDV
Serology
Luminex
ISSN:1743-422X, 1743-422X
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Zusammenfassung:The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we developed and evaluated a Luminex assay for the simultaneous detection of antibodies against CaPV and RVFV in domestic ruminants. In cattle, the assay had a sensitivity (Se) of 98.7% and a specificity (Sp) of 98.3% in detecting anti-LSDV antibodies; both diagnostic parameters were 100% for the detection of anti-RVFV antibodies in this species. In sheep and goats, Se and Sp were 100% for the detection of anti-SPPV and anti-GTPV antibodies while they were 100% and 98.9%, respectively for the detection of anti-RVFV antibody. The assay did not cross react with anti-parapoxvirus antibodies of cattle, sheep, and goats. This multiplex serological assay offers a practical tool for accurate detection and monitoring of the immunological status of domestic ruminant populations against veterinary and socio-economically important capripox- and phleboviral infections, thus has the potential to aid in the strategic application of vaccination programmes.
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ISSN:1743-422X
1743-422X
DOI:10.1186/s12985-024-02602-9