Phenotypic characterisation of bovine alveolar macrophages reveals two major subsets with differential expression of CD163

Bovine alveolar macrophages (AMs) defend the lungs against pathogens such as Mycobacterium bovis ( M. bovis) , the causative agent of bovine tuberculosis. However, little is known about the surface molecules expressed by bovine AMs and whether there is heterogeneity within the population. The purpos...

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Vydáno v:	Scientific reports Ročník 14; číslo 1; s. 14974 - 9
Hlavní autoři:	Randall, Emily M., Sopp, Paul, Raper, Anna, Dry, Inga, Burdon, Tom, Hope, Jayne C., Waddell, Lindsey A.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 28.06.2024 Nature Publishing Group Nature Portfolio
Témata:	631/1647/1407/1492 631/250/2504/342 Alveoli Animals Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - metabolism Bronchoalveolar Lavage Fluid Bronchus Cattle CD14 antigen CD16 antigen CD163 antigen CD40 antigen CD80 antigen CD86 antigen Cell surface CX3CR1 protein Dipeptidyl-peptidase IV Flow Cytometry Genotype & phenotype Heterogeneity Humanities and Social Sciences Immunophenotyping Lavage Lungs Macrophages Macrophages, Alveolar - metabolism Major histocompatibility complex multidisciplinary Mycobacterium bovis - immunology Pathogens Phenotype Phenotypes Population studies Proteins Receptors, Cell Surface - metabolism Science Science (multidisciplinary) Tuberculosis Tuberculosis, Bovine - immunology Tuberculosis, Bovine - metabolism Tuberculosis, Bovine - microbiology
ISSN:	2045-2322, 2045-2322
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Shrnutí:	Bovine alveolar macrophages (AMs) defend the lungs against pathogens such as Mycobacterium bovis ( M. bovis) , the causative agent of bovine tuberculosis. However, little is known about the surface molecules expressed by bovine AMs and whether there is heterogeneity within the population. The purpose of this study was to characterise the bovine AM cell surface phenotype using flow cytometry. Bronchoalveolar lavage samples from four different calves were stained with a combination of antibodies against immune cell molecules prior to flow cytometric analysis. To assess the degree of expression, we considered the distribution and relative intensities of stained and unstained cells. We demonstrated that bovine AMs have high expression of CD172a, ADGRE1, CD206, and CD14, moderate expression of CD80, MHC II, CD1b, and CD40, low expression of CX3CR1 and CD86, and little or no expression of CD16 and CD26. Two distinct subsets of bovine AMs were identified based on CD163 expression. Subsequent analysis showed that the CD163 + subset had greater expression of other typical macrophage molecules compared to the CD163 - subset, suggesting that these cells may perform different roles during infection. The characterisation of the uninfected bovine AM phenotype will provide a foundation for the examination of M. bovis- infected AMs.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-024-65868-7