Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes

Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph...

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Veröffentlicht in:eLife Jg. 11
Hauptverfasser: Yang, Peng, Chen, Yaohui, Huang, Zhiqi, Xia, Huidan, Cheng, Ling, Wu, Hao, Zhang, Yueling, Wang, Fan
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England eLife Sciences Publications Ltd 06.10.2022
eLife Sciences Publications, Ltd
Schlagworte:
Animals
Arthropod Proteins - genetics
differentiation
Gene expression
Genomics
Granulocytes
Hemocytes
Hemolymph
Immune response
Immunity, Innate - genetics
Immunology and Inflammation
Infectious diseases
Innate immunity
invertebrate
Invertebrates
Leukocytes (granulocytic)
Macrophages
Macrophages - metabolism
Mammals - genetics
Monocytes
myeloid cell evolution
NLR Family, Pyrin Domain-Containing 3 Protein - metabolism
Pathogens
Penaeidae - genetics
Penaeus vannamei
Phagocytes
Phagocytes - metabolism
Proteins
Sequence analysis
Sequence Analysis, RNA
Vertebrates
macrophages
differentiation
inflammation
Penaeus vannamei
myeloid cell evolution
invertebrate
immunology
ISSN:2050-084X, 2050-084X
Online-Zugang:Volltext
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Zusammenfassung:Despite the importance of innate immunity in invertebrates, the diversity and function of innate immune cells in invertebrates are largely unknown. Using single-cell RNA-seq, we identified prohemocytes, monocytic hemocytes, and granulocytes as the three major cell-types in the white shrimp hemolymph. Our results identified a novel macrophage-like subset called monocytic hemocytes 2 (MH2) defined by the expression of certain marker genes, including Nlrp3 and Casp1 . This subtype of shrimp hemocytes is phagocytic and expresses markers that indicate some conservation with mammalian macrophages. Combined, our work resolves the heterogenicity of hemocytes in a very economically important aquatic species and identifies a novel innate immune cell subset that is likely a critical player in the immune responses of shrimp to threatening infectious diseases affecting this industry.
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ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.80127