Comparison of infection and human immune responses of two SARS-CoV-2 strains in a humanized hACE2 NIKO mouse model

The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized hACE2 (adenovirus expressing hACE2) NOD-SCID IL2R...

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Vydané v:	Scientific reports Ročník 13; číslo 1; s. 12484 - 10
Hlavní autori:	Yong, Kylie Su Mei, Anderson, Danielle E., Zheng, Adrian Kang Eng, Liu, Min, Tan, Sue Yee, Tan, Wilson Wei Sheng, Chen, Qingfeng, Wang, Lin-Fa
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 01.08.2023 Nature Publishing Group Nature Portfolio
Predmet:	631/250 631/326 Adenoviruses Animal models Animals Antigens Chemokines COVID-19 Cytokines Cytomegalovirus Disease Disease Models, Animal Experimental infection Experiments Global economy Histology Humanities and Social Sciences Humans Immune response Immune system Immunology Infections Inflammation Lung Lung cancer Mice Mice, Inbred NOD Mice, SCID Mice, Transgenic multidisciplinary Mutants Pandemics Pathogenesis Plasmids Proteins SARS-CoV-2 Science Science (multidisciplinary) Severe acute respiratory syndrome coronavirus 2 Spleen Stem cells Therapeutic applications
ISSN:	2045-2322, 2045-2322
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Shrnutí:	The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized hACE2 (adenovirus expressing hACE2) NOD-SCID IL2Rγ −/− (NIKO) mouse model and compare infection with ancestral and mutant (SARS-CoV-2-∆382) strains of SARS-CoV-2. Immune cell infiltration, inflammation, lung damage and pro-inflammatory cytokines and chemokines was observed in humanized hACE2 NIKO mice. Humanized hACE2 NIKO mice infected with the ancestral and mutant SARS-CoV-2 strain had lung inflammation and production of pro-inflammatory cytokines and chemokines. This model can aid in examining the pathological basis of SARS-CoV-2 infection in a human immune environment and evaluation of therapeutic interventions.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-023-39628-y