Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing

Control of CRISPR/Cas12a trans -cleavage is crucial for biosensor development. Here, we show that small circular DNA nanostructures which partially match guide RNA sequences only minimally activate Cas12a ribonucleoproteins. However, linearizing these structures restores activation. Building on this...

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Bibliographic Details
Published in:	Nature communications Vol. 15; no. 1; pp. 1818 - 16
Main Authors:	Deng, Fei, Li, Yi, Yang, Biyao, Sang, Rui, Deng, Wei, Kansara, Maya, Lin, Frank, Thavaneswaran, Subotheni, Thomas, David M., Goldys, Ewa M.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 05.03.2024 Nature Publishing Group Nature Portfolio
Subjects:	631/208/4041/3196 631/45/147 631/45/603 96/10 Amplification Autocatalysis Biosensors Blood circulation Blood plasma Circular DNA Cleavage Colorimetry CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA - genetics DNA, Circular - genetics Gene sequencing gRNA Helicobacter pylori Humanities and Social Sciences multidisciplinary Mutation Nanostructure Nanostructures Nucleic acids Nucleotide sequence Reverse transcription Ribonucleic acid Ribonucleoproteins RNA RNA - genetics RNA, Guide, CRISPR-Cas Systems Room temperature Science Science (multidisciplinary) Severe acute respiratory syndrome coronavirus 2 Single-nucleotide polymorphism
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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