Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients

Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietname...

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Veröffentlicht in:NPJ biofilms and microbiomes Jg. 8; H. 1; S. 87 - 13
Hauptverfasser: Tran, Hoang N. H., Thu, Trang Nguyen Hoang, Nguyen, Phu Huu, Vo, Chi Nguyen, Doan, Khanh Van, Nguyen Ngoc Minh, Chau, Nguyen, Ngoc Tuan, Ta, Van Ngoc Duc, Vu, Khuong An, Hua, Thanh Danh, Nguyen, To Nguyen Thi, Van, Tan Trinh, Pham Duc, Trung, Duong, Ba Lap, Nguyen, Phuc Minh, Hoang, Vinh Chuc, Pham, Duy Thanh, Thwaites, Guy E., Hall, Lindsay J., Slade, Daniel J., Baker, Stephen, Tran, Vinh Hung, Chung The, Hao
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 29.10.2022
Nature Publishing Group
Nature Portfolio
Schlagworte:
631/1647/514/2254
631/326/107
631/326/2565/2134
Asian People
Biomedical and Life Sciences
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - microbiology
Fusobacterium
Fusobacterium - genetics
Gene transfer
Genomics
Horizontal transfer
Humans
Intestinal microflora
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microbiomes
Microbiota
New subspecies
Phylogeny
Polyps
RNA, Ribosomal, 16S - genetics
rRNA 16S
Saliva
Tumors
Virulence factors
ISSN:2055-5008, 2055-5008
Online-Zugang:Volltext
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Zusammenfassung:Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietnamese CRC patients and 25 participants with non-cancerous colorectal polyps to study the colonic microbiomes and genomic diversity of Fusobacterium in this population, using a combination of 16S rRNA gene profiling, anaerobic microbiology, and whole genome analysis. Oral bacteria, including F. nucleatum and Leptotrichia , were significantly more abundant in the tumour microbiomes. We obtained 53 Fusobacterium genomes, representing 26 strains, from the saliva, tumour and non-tumour tissues of six CRC patients. Isolates from the gut belonged to diverse F. nucleatum subspecies ( nucleatum , animalis , vincentii , polymorphum ) and a potential new subspecies of Fusobacterium periodonticum . The Fusobacterium population within each individual was distinct and in some cases diverse, with minimal intra-clonal variation. Phylogenetic analyses showed that within four individuals, tumour-associated Fusobacterium were clonal to those isolated from non-tumour tissues. Genes encoding major virulence factors (Fap2 and RadD) showed evidence of horizontal gene transfer. Our work provides a framework to understand the genomic diversity of Fusobacterium within the CRC patients, which can be exploited for the development of CRC diagnostic and therapeutic options targeting this oncobacterium.
Bibliographie:ObjectType-Article-1
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ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-022-00351-7