Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen

Quantitative hepatitis B core antigen (anti-HBc) measurements could play an important role in evaluating therapeutic outcomes and optimizing the antiviral therapy of chronic hepatitis B infection. In this study, we have developed a simple and rapid fluorescence point-of-care test based on a lateral...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 7; no. 1; pp. 14093 - 8
Main Authors: Liang, Rong-Liang, Deng, Qiao-Ting, Chen, Zhen-Hua, Xu, Xu-Ping, Zhou, Jian-Wei, Liang, Jun-Yu, Dong, Zhi-Ning, Liu, Tian-Cai, Wu, Ying-Song
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 26.10.2017
Nature Publishing Group
Subjects:
631/1647/2196/2197
631/1647/664
631/61/350/59
692/53/2423
692/699/255/234/2513/1549
Antigens
Antiviral agents
Calibration
Chronic infection
Core protein
Europium
Hepatitis
Hepatitis B
Hepatitis B core antigen
Hepatitis B Core Antigens - analysis
Hepatitis B Core Antigens - blood
Hepatitis B Core Antigens - immunology
Humanities and Social Sciences
Humans
Immunoassay
Immunoassay - instrumentation
Immunoassay - methods
Interferon
Microparticles
Microtechnology
multidisciplinary
Organometallic Compounds
Science
Science (multidisciplinary)
Sensitivity and Specificity
Statistical analysis
Time Factors
ISSN:2045-2322, 2045-2322
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Quantitative hepatitis B core antigen (anti-HBc) measurements could play an important role in evaluating therapeutic outcomes and optimizing the antiviral therapy of chronic hepatitis B infection. In this study, we have developed a simple and rapid fluorescence point-of-care test based on a lateral flow immunoassay (LFIA) method integrated with Eu (III) chelate microparticles to quantitatively determine anti-HBc concentrations in serum. This assay is based on a direct competitive immunoassay performed on lateral flow test strips with an assay time of 15 min. The Eu (III) chelate microparticle-based LFIA assay could quantitatively detect anti-HBc levels with a limit of detection of 0.31 IU mL −1 , and exhibited a wide linear range (0.63–640 IU mL −1 ). The intra- and inter-assay coefficients of variation for anti-HBc were both less than 10% and a satisfactory dilution test and accuracy were demonstrated. There were no statistically significant differences in sensitivity or specificity in serum samples between the Eu (III) chelate microparticle-based LFIA strips and the Abbott Architect kit. A simple, rapid and effective quantitative detection of anti-HBc was possible using the Eu (III) chelate microparticle-based LFIA strips. The strips will provide diagnostic value for clinical application.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-14427-4