Europium (III) chelate microparticle-based lateral flow immunoassay strips for rapid and quantitative detection of antibody to hepatitis B core antigen

Quantitative hepatitis B core antigen (anti-HBc) measurements could play an important role in evaluating therapeutic outcomes and optimizing the antiviral therapy of chronic hepatitis B infection. In this study, we have developed a simple and rapid fluorescence point-of-care test based on a lateral...

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Vydané v:Scientific reports Ročník 7; číslo 1; s. 14093 - 8
Hlavní autori: Liang, Rong-Liang, Deng, Qiao-Ting, Chen, Zhen-Hua, Xu, Xu-Ping, Zhou, Jian-Wei, Liang, Jun-Yu, Dong, Zhi-Ning, Liu, Tian-Cai, Wu, Ying-Song
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 26.10.2017
Nature Publishing Group
Predmet:
631/1647/2196/2197
631/1647/664
631/61/350/59
692/53/2423
692/699/255/234/2513/1549
Antigens
Antiviral agents
Calibration
Chronic infection
Core protein
Europium
Hepatitis
Hepatitis B
Hepatitis B core antigen
Hepatitis B Core Antigens - analysis
Hepatitis B Core Antigens - blood
Hepatitis B Core Antigens - immunology
Humanities and Social Sciences
Humans
Immunoassay
Immunoassay - instrumentation
Immunoassay - methods
Interferon
Microparticles
Microtechnology
multidisciplinary
Organometallic Compounds
Science
Science (multidisciplinary)
Sensitivity and Specificity
Statistical analysis
Time Factors
ISSN:2045-2322, 2045-2322
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Shrnutí:Quantitative hepatitis B core antigen (anti-HBc) measurements could play an important role in evaluating therapeutic outcomes and optimizing the antiviral therapy of chronic hepatitis B infection. In this study, we have developed a simple and rapid fluorescence point-of-care test based on a lateral flow immunoassay (LFIA) method integrated with Eu (III) chelate microparticles to quantitatively determine anti-HBc concentrations in serum. This assay is based on a direct competitive immunoassay performed on lateral flow test strips with an assay time of 15 min. The Eu (III) chelate microparticle-based LFIA assay could quantitatively detect anti-HBc levels with a limit of detection of 0.31 IU mL −1 , and exhibited a wide linear range (0.63–640 IU mL −1 ). The intra- and inter-assay coefficients of variation for anti-HBc were both less than 10% and a satisfactory dilution test and accuracy were demonstrated. There were no statistically significant differences in sensitivity or specificity in serum samples between the Eu (III) chelate microparticle-based LFIA strips and the Abbott Architect kit. A simple, rapid and effective quantitative detection of anti-HBc was possible using the Eu (III) chelate microparticle-based LFIA strips. The strips will provide diagnostic value for clinical application.
Bibliografia:ObjectType-Article-1
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-14427-4