Highly diverse flavobacterial phages isolated from North Sea spring blooms

It is generally recognized that phages are a mortality factor for their bacterial hosts. This could be particularly true in spring phytoplankton blooms, which are known to be closely followed by a highly specialized bacterial community. We hypothesized that phages modulate these dense heterotrophic...

Full description

Saved in:
Bibliographic Details
Published in:The ISME Journal Vol. 16; no. 2; pp. 555 - 568
Main Authors: Bartlau, Nina, Wichels, Antje, Krohne, Georg, Adriaenssens, Evelien M., Heins, Anneke, Fuchs, Bernhard M., Amann, Rudolf, Moraru, Cristina
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.02.2022
Oxford University Press
Subjects:
45/22
45/23
631/326/1321
631/326/171/1878
Bacteria
Bacteriophages - genetics
Biodegradation
Biomedical and Life Sciences
CRISPR
Ecology
Eutrophication
Evolutionary Biology
Flavobacteriaceae - genetics
Genera
Genomic analysis
Heterotrophic bacteria
Humans
Life Sciences
Metagenome
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Microorganisms
New families
New species
North Sea
Phages
Phytoplankton
Plankton
Polysaccharides
Saccharides
North Sea
ISSN:1751-7362, 1751-7370, 1751-7370
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It is generally recognized that phages are a mortality factor for their bacterial hosts. This could be particularly true in spring phytoplankton blooms, which are known to be closely followed by a highly specialized bacterial community. We hypothesized that phages modulate these dense heterotrophic bacteria successions following phytoplankton blooms. In this study, we focused on Flavobacteriia , because they are main responders during these blooms and have an important role in the degradation of polysaccharides. A cultivation-based approach was used, obtaining 44 lytic flavobacterial phages (flavophages), representing twelve new species from two viral realms. Taxonomic analysis allowed us to delineate ten new phage genera and ten new families, from which nine and four, respectively, had no previously cultivated representatives. Genomic analysis predicted various life styles and genomic replication strategies. A likely eukaryote-associated host habitat was reflected in the gene content of some of the flavophages. Detection in cellular metagenomes and by direct-plating showed that part of these phages were actively replicating in the environment during the 2018 spring bloom. Furthermore, CRISPR/Cas spacers and re-isolation during two consecutive years suggested that, at least part of the new flavophages are stable components of the microbial community in the North Sea. Together, our results indicate that these diverse flavophages have the potential to modulate their respective host populations.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1751-7362
1751-7370
1751-7370
DOI:10.1038/s41396-021-01097-4