Cellular anatomy of the mouse primary motor cortex

An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated and interpreted 1 . Here, as part of the collaborative Brain Initiative Cell Census Ne...

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Published in:Nature (London) Vol. 598; no. 7879; pp. 159 - 166
Main Authors: Muñoz-Castañeda, Rodrigo, Zingg, Brian, Matho, Katherine S., Chen, Xiaoyin, Wang, Quanxin, Foster, Nicholas N., Li, Anan, Narasimhan, Arun, Hirokawa, Karla E., Huo, Bingxing, Bannerjee, Samik, Korobkova, Laura, Park, Chris Sin, Park, Young-Gyun, Bienkowski, Michael S., Chon, Uree, Wheeler, Diek W., Li, Xiangning, Wang, Yun, Naeemi, Maitham, Xie, Peng, Liu, Lijuan, Kelly, Kathleen, An, Xu, Attili, Sarojini M., Bowman, Ian, Bludova, Anastasiia, Cetin, Ali, Ding, Liya, Drewes, Rhonda, D’Orazi, Florence, Elowsky, Corey, Fischer, Stephan, Galbavy, William, Gao, Lei, Gillis, Jesse, Groblewski, Peter A., Gou, Lin, Hahn, Joel D., Hatfield, Joshua T., Hintiryan, Houri, Huang, Junxiang Jason, Kondo, Hideki, Kuang, Xiuli, Lesnar, Philip, Li, Xu, Li, Yaoyao, Lin, Mengkuan, Lo, Darrick, Mizrachi, Judith, Mok, Stephanie, Nicovich, Philip R., Palaniswamy, Ramesh, Palmer, Jason, Qi, Xiaoli, Shen, Elise, Sun, Yu-Chi, Tao, Huizhong W., Wakemen, Wayne, Wang, Yimin, Yao, Shenqin, Yuan, Jing, Zhan, Huiqing, Zhu, Muye, Ng, Lydia, Zhang, Li I., Lim, Byung Kook, Hawrylycz, Michael, Gong, Hui, Gee, James C., Kim, Yongsoo, Chung, Kwanghun, Yang, X. William, Peng, Hanchuan, Luo, Qingming, Mitra, Partha P., Zador, Anthony M., Zeng, Hongkui, Ascoli, Giorgio A., Josh Huang, Z., Osten, Pavel, Harris, Julie A., Dong, Hong-Wei
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 07.10.2021
Nature Publishing Group
Subjects:
14/1
14/19
14/34
14/63
14/69
631/378/2632/1663
631/378/3920
Anatomy
Animals
Atlases as Topic
Bioinformatics
Brain
Brain architecture
Cellular structure
Circuits
Collaboration
Cortex (motor)
Datasets
Female
GABAergic Neurons - cytology
GABAergic Neurons - metabolism
Gene expression
Glutamates - metabolism
Humanities and Social Sciences
Image reconstruction
Labeling
Laboratories
Male
Medical imaging
Mice
Mice, Inbred C57BL
Motor Cortex - anatomy & histology
Motor Cortex - cytology
Motor task performance
multidisciplinary
Neuroimaging
Neurons
Neurons - classification
Neurons - cytology
Neurons - metabolism
Organ Specificity
Science
Science (multidisciplinary)
Sequence Analysis, RNA
Single-Cell Analysis
Spinal cord
Tropical diseases
Wiring
ISSN:0028-0836, 1476-4687, 1476-4687
Online Access:Get full text
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Summary:An essential step toward understanding brain function is to establish a structural framework with cellular resolution on which multi-scale datasets spanning molecules, cells, circuits and systems can be integrated and interpreted 1 . Here, as part of the collaborative Brain Initiative Cell Census Network (BICCN), we derive a comprehensive cell type-based anatomical description of one exemplar brain structure, the mouse primary motor cortex, upper limb area (MOp-ul). Using genetic and viral labelling, barcoded anatomy resolved by sequencing, single-neuron reconstruction, whole-brain imaging and cloud-based neuroinformatics tools, we delineated the MOp-ul in 3D and refined its sublaminar organization. We defined around two dozen projection neuron types in the MOp-ul and derived an input–output wiring diagram, which will facilitate future analyses of motor control circuitry across molecular, cellular and system levels. This work provides a roadmap towards a comprehensive cellular-resolution description of mammalian brain architecture. Multi-modal analysis is used to generate a 3D atlas of the upper limb area of the mouse primary motor cortex, providing a framework for future studies of motor control circuitry.
Bibliography:ObjectType-Article-1
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ISSN:0028-0836
1476-4687
1476-4687
DOI:10.1038/s41586-021-03970-w