A Versatile Sample Processing Workflow for Metagenomic Pathogen Detection

Metagenomics is currently the only generic method for pathogen detection. Starting from RNA allows the assessment of the whole sample community including RNA viruses. Here we present our modular concerted protocol for sample processing for diagnostic metagenomics analysis of human, animal, and food...

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Veröffentlicht in:Scientific reports Jg. 8; H. 1; S. 13108 - 12
Hauptverfasser: Wylezich, Claudia, Papa, Anna, Beer, Martin, Höper, Dirk
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 30.08.2018
Nature Publishing Group
Schlagworte:
45/90
45/91
692/699/255/1318
692/699/255/1715
692/699/255/2514
Animal human relations
Humanities and Social Sciences
multidisciplinary
Pathogens
RNA viruses
Science
Science (multidisciplinary)
ISSN:2045-2322, 2045-2322
Online-Zugang:Volltext
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Zusammenfassung:Metagenomics is currently the only generic method for pathogen detection. Starting from RNA allows the assessment of the whole sample community including RNA viruses. Here we present our modular concerted protocol for sample processing for diagnostic metagenomics analysis of human, animal, and food samples. The workflow does not rely on dedicated amplification steps at any stage in the process and, in contrast to published methods, libraries prepared accordingly will yield only minute amounts of unclassifiable reads. We confirmed the performance of the approach using a spectrum of pathogen/matrix-combinations showing it has the potential to become a commonly usable analytical framework.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-31496-1