A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation

There is accumulating evidence that the lower airway microbiota impacts lung health. However, the link between microbial community composition and lung homeostasis remains elusive. We combine amplicon sequencing and bacterial culturing to characterize the viable bacterial community in 234 longitudin...

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Vydané v:	Nature communications Ročník 12; číslo 1; s. 2126 - 17
Hlavní autori:	Das, Sudip, Bernasconi, Eric, Koutsokera, Angela, Wurlod, Daniel-Adrien, Tripathi, Vishwachi, Bonilla-Rosso, Germán, Aubert, John-David, Derkenne, Marie-France, Mercier, Louis, Pattaroni, Céline, Rapin, Alexis, von Garnier, Christophe, Marsland, Benjamin J., Engel, Philipp, Nicod, Laurent P.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 09.04.2021 Nature Publishing Group Nature Portfolio
Predmet:	38 45 45/23 45/77 631/158/855 631/250/1854 631/250/2504 631/250/256 631/326 Adult Allografts - immunology Allografts - microbiology Alveoli Bacteria Bacteria - genetics Bacteria - immunology Bacteria - isolation & purification Bacteria - pathogenicity Bacterial Load - immunology Bacteriological Techniques Bioinformatics Bronchoalveolar Lavage Fluid - microbiology Bronchoscopy Bronchus Community composition DNA, Bacterial - isolation & purification Ecological balance Ecology Female Gene expression Graft rejection Graft Rejection - diagnosis Graft Rejection - immunology Graft Rejection - microbiology Health risks Homeostasis Humanities and Social Sciences Humans Immune Tolerance Immunological tolerance Lavage Longitudinal Studies Lung - immunology Lung - microbiology Lung transplantation Lung Transplantation - adverse effects Lung transplants Lungs Male Metagenomics Microbiomes Microbiota Microbiota - genetics Microbiota - immunology Microorganisms Middle Aged multidisciplinary Pneumonia, Bacterial - diagnosis Pneumonia, Bacterial - immunology Pneumonia, Bacterial - microbiology Prospective Studies Respiratory function RNA, Ribosomal, 16S - genetics rRNA Science Science (multidisciplinary) Stability Transplantation
ISSN:	2041-1723, 2041-1723
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Shrnutí:	There is accumulating evidence that the lower airway microbiota impacts lung health. However, the link between microbial community composition and lung homeostasis remains elusive. We combine amplicon sequencing and bacterial culturing to characterize the viable bacterial community in 234 longitudinal bronchoalveolar lavage samples from 64 lung transplant recipients and establish links to viral loads, host gene expression, lung function, and transplant health. We find that the lung microbiota post-transplant can be categorized into four distinct compositional states, ‘pneumotypes’. The predominant ‘balanced’ pneumotype is characterized by a diverse bacterial community with moderate viral loads, and host gene expression profiles suggesting immune tolerance. The other three pneumotypes are characterized by being either microbiota-depleted, or dominated by potential pathogens, and are linked to increased immune activity, lower respiratory function, and increased risks of infection and rejection. Collectively, our findings establish a link between the lung microbial ecosystem, human lung function, and clinical stability post-transplant. Here, the authors combine 16 S rRNA sequencing, culture and bioinformatics to profile the microbiome in 234 serial bronchoalveolar lavage samples from 64 lung transplant recipients collected over 49-months and identify distinct compositional states, termed pneumotypes, linked to current health status, and establish a collection of primary lung bacterial isolates, LuMiCol.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Undefined-3
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-021-22344-4