High-resolution label-free 3D mapping of extracellular pH of single living cells

Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level...

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Vydáno v:	Nature communications Ročník 10; číslo 1; s. 5610 - 9
Hlavní autoři:	Zhang, Yanjun, Takahashi, Yasufumi, Hong, Sung Pil, Liu, Fengjie, Bednarska, Joanna, Goff, Philip S., Novak, Pavel, Shevchuk, Andrew, Gopal, Sahana, Barozzi, Iros, Magnani, Luca, Sakai, Hideki, Suguru, Yoshimoto, Fujii, Takuto, Erofeev, Alexander, Gorelkin, Peter, Majouga, Alexander, Weiss, Dominik J., Edwards, Christopher, Ivanov, Aleksandar P., Klenerman, David, Sviderskaya, Elena V., Edel, Joshua B., Korchev, Yuri
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 06.12.2019 Nature Publishing Group Nature Portfolio
Témata:	13 14 14/10 631/57/2265 631/57/2282 639/638 639/925 Barrel plating Biophysics Breast cancer Cancer Cell Line, Tumor Conductance Diatoms - cytology Heterogeneity Humanities and Social Sciences Humans Hydrogen-Ion Concentration Imaging, Three-Dimensional - methods Mapping Melanoma Microscopy, Electron, Scanning multidisciplinary Neoplasms - diagnostic imaging Neoplasms - pathology pH effects Response time Science Science (multidisciplinary) Sensitivity Single-Cell Analysis - methods Spatial resolution Temporal resolution Tumors
ISSN:	2041-1723, 2041-1723
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Abstract	Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.
AbstractList	Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells. Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells. Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.
ArticleNumber	5610
Author	Suguru, Yoshimoto Gorelkin, Peter Zhang, Yanjun Gopal, Sahana Hong, Sung Pil Fujii, Takuto Takahashi, Yasufumi Sviderskaya, Elena V. Weiss, Dominik J. Korchev, Yuri Bednarska, Joanna Klenerman, David Novak, Pavel Sakai, Hideki Liu, Fengjie Majouga, Alexander Goff, Philip S. Barozzi, Iros Erofeev, Alexander Magnani, Luca Ivanov, Aleksandar P. Shevchuk, Andrew Edwards, Christopher Edel, Joshua B.
Author_xml	– sequence: 1 givenname: Yanjun surname: Zhang fullname: Zhang, Yanjun email: yanjun.zhang@imperial.ac.uk organization: Department of Medicine, Imperial College London, Tianjin Neurological Institute, Tianjin Medical University General Hospital – sequence: 2 givenname: Yasufumi orcidid: 0000-0003-2834-8300 surname: Takahashi fullname: Takahashi, Yasufumi organization: Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST) – sequence: 3 givenname: Sung Pil surname: Hong fullname: Hong, Sung Pil organization: Department of Surgery and Cancer, Imperial College London – sequence: 4 givenname: Fengjie surname: Liu fullname: Liu, Fengjie organization: Department of Earth Science & Engineering, Imperial College London – sequence: 5 givenname: Joanna surname: Bednarska fullname: Bednarska, Joanna organization: Department of Medicine, Imperial College London – sequence: 6 givenname: Philip S. orcidid: 0000-0003-4371-5527 surname: Goff fullname: Goff, Philip S. organization: Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George’s, University of London – sequence: 7 givenname: Pavel surname: Novak fullname: Novak, Pavel organization: Department of Medicine, Imperial College London, National University of Science and Technology “MISIS” – sequence: 8 givenname: Andrew surname: Shevchuk fullname: Shevchuk, Andrew organization: Department of Medicine, Imperial College London – sequence: 9 givenname: Sahana surname: Gopal fullname: Gopal, Sahana organization: Department of Medicine, Imperial College London – sequence: 10 givenname: Iros surname: Barozzi fullname: Barozzi, Iros organization: Department of Surgery and Cancer, Imperial College London – sequence: 11 givenname: Luca orcidid: 0000-0002-7534-0785 surname: Magnani fullname: Magnani, Luca organization: Department of Surgery and Cancer, Imperial College London – sequence: 12 givenname: Hideki surname: Sakai fullname: Sakai, Hideki organization: Department of Pharmaceutical Physiology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama – sequence: 13 givenname: Yoshimoto surname: Suguru fullname: Suguru, Yoshimoto organization: Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi – sequence: 14 givenname: Takuto surname: Fujii fullname: Fujii, Takuto organization: Department of Pharmaceutical Physiology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama – sequence: 15 givenname: Alexander surname: Erofeev fullname: Erofeev, Alexander organization: National University of Science and Technology “MISIS”, Department of Chemistry, Lomonosov Moscow State University – sequence: 16 givenname: Peter surname: Gorelkin fullname: Gorelkin, Peter organization: National University of Science and Technology “MISIS” – sequence: 17 givenname: Alexander surname: Majouga fullname: Majouga, Alexander organization: Department of Chemistry, Lomonosov Moscow State University – sequence: 18 givenname: Dominik J. surname: Weiss fullname: Weiss, Dominik J. organization: Department of Earth Science & Engineering, Imperial College London – sequence: 19 givenname: Christopher surname: Edwards fullname: Edwards, Christopher organization: Department of Medicine, Imperial College London – sequence: 20 givenname: Aleksandar P. orcidid: 0000-0003-1419-1381 surname: Ivanov fullname: Ivanov, Aleksandar P. organization: Department of Chemistry, Imperial College London – sequence: 21 givenname: David surname: Klenerman fullname: Klenerman, David organization: Department of Chemistry, University of Cambridge – sequence: 22 givenname: Elena V. orcidid: 0000-0002-4177-8236 surname: Sviderskaya fullname: Sviderskaya, Elena V. email: esviders@sgul.ac.uk organization: Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George’s, University of London – sequence: 23 givenname: Joshua B. orcidid: 0000-0001-5870-8659 surname: Edel fullname: Edel, Joshua B. email: joshua.edel@imperial.ac.uk organization: Department of Chemistry, Imperial College London – sequence: 24 givenname: Yuri surname: Korchev fullname: Korchev, Yuri email: y.korchev@imperial.ac.uk organization: Department of Medicine, Imperial College London, Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31811139$$D View this record in MEDLINE/PubMed
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Snippet	Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with... Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H in cellular and subcellular processes, with... Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with... Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of...
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SubjectTerms	13 14 14/10 631/57/2265 631/57/2282 639/638 639/925 Barrel plating Biophysics Breast cancer Cancer Cell Line, Tumor Conductance Diatoms - cytology Heterogeneity Humanities and Social Sciences Humans Hydrogen-Ion Concentration Imaging, Three-Dimensional - methods Mapping Melanoma Microscopy, Electron, Scanning multidisciplinary Neoplasms - diagnostic imaging Neoplasms - pathology pH effects Response time Science Science (multidisciplinary) Sensitivity Single-Cell Analysis - methods Spatial resolution Temporal resolution Tumors
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Title	High-resolution label-free 3D mapping of extracellular pH of single living cells
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