High-resolution label-free 3D mapping of extracellular pH of single living cells

Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level...

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Vydáno v:	Nature communications Ročník 10; číslo 1; s. 5610 - 9
Hlavní autoři:	Zhang, Yanjun, Takahashi, Yasufumi, Hong, Sung Pil, Liu, Fengjie, Bednarska, Joanna, Goff, Philip S., Novak, Pavel, Shevchuk, Andrew, Gopal, Sahana, Barozzi, Iros, Magnani, Luca, Sakai, Hideki, Suguru, Yoshimoto, Fujii, Takuto, Erofeev, Alexander, Gorelkin, Peter, Majouga, Alexander, Weiss, Dominik J., Edwards, Christopher, Ivanov, Aleksandar P., Klenerman, David, Sviderskaya, Elena V., Edel, Joshua B., Korchev, Yuri
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 06.12.2019 Nature Publishing Group Nature Portfolio
Témata:	13 14 14/10 631/57/2265 631/57/2282 639/638 639/925 Barrel plating Biophysics Breast cancer Cancer Cell Line, Tumor Conductance Diatoms - cytology Heterogeneity Humanities and Social Sciences Humans Hydrogen-Ion Concentration Imaging, Three-Dimensional - methods Mapping Melanoma Microscopy, Electron, Scanning multidisciplinary Neoplasms - diagnostic imaging Neoplasms - pathology pH effects Response time Science Science (multidisciplinary) Sensitivity Single-Cell Analysis - methods Spatial resolution Temporal resolution Tumors
ISSN:	2041-1723, 2041-1723
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Shrnutí:	Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H + in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Current methods to measure extracellular pH are often limited in resolution and response times. Here the authors present a label-free nanoprobe, consisting of a zwitterionic nanomembrane at the tip of a nanopipette, which enables high spatiotemporal resolution pH measurements and topography-pH 3D mapping in live cancer cells.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2041-1723 2041-1723
DOI:	10.1038/s41467-019-13535-1