A coordinated progression of progenitor cell states initiates urinary tract development

The kidney and upper urinary tract develop through reciprocal interactions between the ureteric bud and the surrounding mesenchyme. Ureteric bud branching forms the arborized collecting duct system of the kidney, while ureteric tips promote nephron formation from dedicated progenitor cells. While ne...

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Vydané v:Nature communications Ročník 12; číslo 1; s. 2627 - 16
Hlavní autori: Sanchez-Ferras, Oraly, Pacis, Alain, Sotiropoulou, Maria, Zhang, Yuhong, Wang, Yu Chang, Bourgey, Mathieu, Bourque, Guillaume, Ragoussis, Jiannis, Bouchard, Maxime
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 11.05.2021
Nature Publishing Group
Nature Portfolio
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Animals
AP-2 protein
Cell Differentiation - genetics
Cells (biology)
Clusters
Collecting duct
Developmental disabilities
Embryo, Mammalian
Embryos
Female
GATA-3 protein
GATA3 Transcription Factor - metabolism
Gene Expression Regulation, Developmental
Humanities and Social Sciences
Kidneys
Male
Mesenchyme
Mesoderm
Mice
Mice, Transgenic
Models, Animal
Morphogenesis
multidisciplinary
Nephrons - embryology
Organogenesis - genetics
Populations
Progenitor cells
Ribonucleic acid
RNA
RNA-Seq
Science
Science (multidisciplinary)
Single-Cell Analysis
Stem cells
Stem Cells - physiology
Tips
Transcription Factor AP-2 - metabolism
Transcription factors
Ureter
Urinary tract
Urogenital system
ISSN:2041-1723, 2041-1723
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Shrnutí:The kidney and upper urinary tract develop through reciprocal interactions between the ureteric bud and the surrounding mesenchyme. Ureteric bud branching forms the arborized collecting duct system of the kidney, while ureteric tips promote nephron formation from dedicated progenitor cells. While nephron progenitor cells are relatively well characterized, the origin of ureteric bud progenitors has received little attention so far. It is well established that the ureteric bud is induced from the nephric duct, an epithelial duct derived from the intermediate mesoderm of the embryo. However, the cell state transitions underlying the progression from intermediate mesoderm to nephric duct and ureteric bud remain unknown. Here we show that nephric duct morphogenesis results from the coordinated organization of four major progenitor cell populations. Using single cell RNA-seq and Cluster RNA-seq, we show that these progenitors emerge in time and space according to a stereotypical pattern. We identify the transcription factors Tfap2a/b and Gata3 as critical coordinators of this progenitor cell progression. This study provides a better understanding of the cellular origin of the renal collecting duct system and associated urinary tract developmental diseases, which may inform guided differentiation of functional kidney tissue. Nephric duct (ND)-derived ureteric buds (UB) form the kidney collecting duct system, while ureteric tips promote nephron formation. Here the authors use single-cell RNA-seq and introduce Cluster RNA-seq to identify four progenitor populations in developing ND/UB regulated by the transcription factors Tfap2a/b and Gata3.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-22931-5