Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam

HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented....

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Vydáno v:	Scientific reports Ročník 8; číslo 1; s. 6833 - 8
Hlavní autoři:	Linster, Martin, Do, Lien Anh Ha, Minh, Ngo Ngoc Quang, Chen, Yihui, Zhe, Zhu, Tuan, Tran Anh, Tuan, Ha Manh, Su, Yvonne C. F., van Doorn, H. Rogier, Moorthy, Mahesh, Smith, Gavin J. D.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.05.2018 Nature Publishing Group Nature Portfolio
Témata:	45/23 45/77 631/181/757 692/699/255/2514 Acute Disease Adolescent Chi-Square Distribution Child Child, Preschool Children Epidemiology Female Follow-Up Studies Gene flow Genetic analysis Genetic diversity Genetic Variation Genome, Viral Genomes Humanities and Social Sciences Humans Infant Infant, Newborn Male Molecular Epidemiology - methods multidisciplinary Parainfluenza Parainfluenza Virus 1, Human - genetics Parainfluenza Virus 2, Human - genetics Parainfluenza Virus 3, Human - genetics Parainfluenza Virus 4, Human - genetics Phylogenetics Phylogeny Real-Time Polymerase Chain Reaction Respiratory Tract Infections - epidemiology Respirovirus Infections - epidemiology Science Science (multidisciplinary) Severity of Illness Index Statistics, Nonparametric Vietnam - epidemiology Whole Genome Sequencing Vietnam
ISSN:	2045-2322, 2045-2322
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Shrnutí:	HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented. Respiratory samples were screened for HPIV1–4 by real-time PCR. Demographic and clinical data of patients infected with different HPIV were compared. We used a hemi-nested PCR approach to generate viral genome sequences from HPIV-positive samples and conducted a comprehensive phylogenetic analysis. In total, 170 samples tested positive for HPIV. HPIV3 was most commonly detected in our cohort and 80 co-detections of HPIV with other respiratory viruses were found. Phylogenetic analyses suggest local endemic circulation as well as punctuated introductions of new HPIV lineages. Viral gene flow analysis revealed that Viet Nam is a net importer of viral genetic diversity. Epidemiological analyses imply similar disease severity for all HPIV species. HPIV sequences from Viet Nam formed local clusters and were interspersed with sequences from diverse geographic regions. Combined, this new knowledge will help to investigate global HPIV circulation patterns in more detail and ultimately define more suitable vaccine strains.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-018-24767-4