Clinical and Molecular Epidemiology of Human Parainfluenza Viruses 1–4 in Children from Viet Nam

HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented....

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports Vol. 8; no. 1; pp. 6833 - 8
Main Authors: Linster, Martin, Do, Lien Anh Ha, Minh, Ngo Ngoc Quang, Chen, Yihui, Zhe, Zhu, Tuan, Tran Anh, Tuan, Ha Manh, Su, Yvonne C. F., van Doorn, H. Rogier, Moorthy, Mahesh, Smith, Gavin J. D.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 01.05.2018
Nature Publishing Group
Nature Portfolio
Subjects:
45/23
45/77
631/181/757
692/699/255/2514
Acute Disease
Adolescent
Chi-Square Distribution
Child
Child, Preschool
Children
Epidemiology
Female
Follow-Up Studies
Gene flow
Genetic analysis
Genetic diversity
Genetic Variation
Genome, Viral
Genomes
Humanities and Social Sciences
Humans
Infant
Infant, Newborn
Male
Molecular Epidemiology - methods
multidisciplinary
Parainfluenza
Parainfluenza Virus 1, Human - genetics
Parainfluenza Virus 2, Human - genetics
Parainfluenza Virus 3, Human - genetics
Parainfluenza Virus 4, Human - genetics
Phylogenetics
Phylogeny
Real-Time Polymerase Chain Reaction
Respiratory Tract Infections - epidemiology
Respirovirus Infections - epidemiology
Science
Science (multidisciplinary)
Severity of Illness Index
Statistics, Nonparametric
Vietnam - epidemiology
Whole Genome Sequencing
Vietnam
ISSN:2045-2322, 2045-2322
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:HPIVs are serologically and genetically grouped into four species that account for up to 10% of all hospitalizations due to acute respiratory infection in children under the age of five. Genetic and epidemiological data for the four HPIVs derived from two pediatric cohorts in Viet Nam are presented. Respiratory samples were screened for HPIV1–4 by real-time PCR. Demographic and clinical data of patients infected with different HPIV were compared. We used a hemi-nested PCR approach to generate viral genome sequences from HPIV-positive samples and conducted a comprehensive phylogenetic analysis. In total, 170 samples tested positive for HPIV. HPIV3 was most commonly detected in our cohort and 80 co-detections of HPIV with other respiratory viruses were found. Phylogenetic analyses suggest local endemic circulation as well as punctuated introductions of new HPIV lineages. Viral gene flow analysis revealed that Viet Nam is a net importer of viral genetic diversity. Epidemiological analyses imply similar disease severity for all HPIV species. HPIV sequences from Viet Nam formed local clusters and were interspersed with sequences from diverse geographic regions. Combined, this new knowledge will help to investigate global HPIV circulation patterns in more detail and ultimately define more suitable vaccine strains.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-018-24767-4