Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing

Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a to...

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Veröffentlicht in:Nature communications Jg. 12; H. 1; S. 1657 - 13
Hauptverfasser: Hu, Yangjie, Omary, Moutasem, Hu, Yun, Doron, Ohad, Hoermayer, Lukas, Chen, Qingguo, Megides, Or, Chekli, Ori, Ding, Zhaojun, Friml, Jiří, Zhao, Yunde, Tsarfaty, Ilan, Shani, Eilon
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 12.03.2021
Nature Publishing Group
Nature Portfolio
Schlagworte:
13
14
14/10
14/19
38
631/449/1741/1576
631/449/2653/1974
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - metabolism
Biosynthesis
Cytology
Flow mapping
Growth conditions
Humanities and Social Sciences
Indoleacetic Acids - metabolism
Kinetics
Meristem - metabolism
Meristems
Morphology
multidisciplinary
Oxygenases - metabolism
Plant Development
Plant growth
Plant roots
Plant Roots - cytology
Plant Roots - growth & development
Plant Roots - metabolism
Regional development
Root development
Science
Science (multidisciplinary)
Stability
Temporal resolution
Tracking
ISSN:2041-1723, 2041-1723
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Zusammenfassung:Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the root and refine the contributions of key players in this process. In addition, we determine the quantitative kinetics of Arabidopsis root meristem skewing, which depends on local auxin gradients but does not require PIN2 and AUX1 auxin transporter activities. Beyond the mechanistic insights into root development, the tools developed here will enable biologists to study kinetics and morphology of various critical processes at the single cell-level in whole organisms. Auxin gradients regulate plant root growth and development. Here the authors manipulate auxin synthesis in specific root cell types and use single-cell nucleus tracking and morphokinetics to map directional auxin flow in the root and quantify the kinetics of meristem skewing.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-21802-3