Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine

The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also known...

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Vydáno v:	Cellular & molecular immunology Ročník 17; číslo 6; s. 613 - 620
Hlavní autoři:	Tai, Wanbo, He, Lei, Zhang, Xiujuan, Pu, Jing, Voronin, Denis, Jiang, Shibo, Zhou, Yusen, Du, Lanying
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	China Nature Publishing Group 01.06.2020
Témata:	ACE2 Amino Acid Sequence Angiotensin Angiotensin-converting enzyme 2 Animals Antibodies Antibodies, Viral - immunology Antisera Betacoronavirus - immunology Betacoronavirus - metabolism Binding Sites Chiroptera Coronaviridae Coronavirus Infections - immunology Coronavirus Infections - metabolism Coronavirus Infections - prevention & control Coronavirus Infections - therapy Coronavirus Infections - virology Coronaviruses COVID-19 COVID-19 Vaccines Cross Reactions HEK293 Cells Humans Infections Mice Pandemics Peptidyl-dipeptidase A Pneumonia, Viral - immunology Pneumonia, Viral - metabolism Pneumonia, Viral - therapy Pneumonia, Viral - virology Protein Binding Proteins Public health Receptor, Angiotensin, Type 2 - metabolism SARS Virus - immunology SARS Virus - metabolism SARS-CoV-2 Sequence Alignment Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - metabolism Vaccines Viral Vaccines - immunology Viral Vaccines - metabolism Virus attachment Virus Internalization SARS-CoV-2 cross-neutralization 2019 novel coronavirus viral inhibitor receptor-binding domain spike protein
ISSN:	1672-7681, 2042-0226, 2042-0226
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Shrnutí:	The outbreak of Coronavirus Disease 2019 (COVID-19) has posed a serious threat to global public health, calling for the development of safe and effective prophylactics and therapeutics against infection of its causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also known as 2019 novel coronavirus (2019-nCoV). The CoV spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Here, we identified the receptor-binding domain (RBD) in SARS-CoV-2 S protein and found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibited significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD and could block the binding and, hence, attachment of SARS-CoV-2 RBD and SARS-CoV RBD to ACE2-expressing cells, thus inhibiting their infection to host cells. SARS-CoV RBD-specific antibodies could cross-react with SARS-CoV-2 RBD protein, and SARS-CoV RBD-induced antisera could cross-neutralize SARS-CoV-2, suggesting the potential to develop SARS-CoV RBD-based vaccines for prevention of SARS-CoV-2 and SARS-CoV infection.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	1672-7681 2042-0226 2042-0226
DOI:	10.1038/s41423-020-0400-4