Evaluation of lockdown effect on SARS-CoV-2 dynamics through viral genome quantification in waste water, Greater Paris, France, 5 March to 23 April 2020

IntroductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of coronavirus disease (COVID-19). People infected with SARS-CoV-2 may exhibit no or mild non-specific symptoms; thus, they may contribute to silent circulation of the virus among humans. Since SARS-CoV...

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Veröffentlicht in:Euro surveillance : bulletin européen sur les maladies transmissibles Jg. 25; H. 50; S. 38
Hauptverfasser: Wurtzer, S, Marechal, V, Mouchel, JM, Maday, Y, Teyssou, R, Richard, E, Almayrac, JL, Moulin, L
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Sweden Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) 17.12.2020
European Centre for Disease Prevention and Control (ECDC)
Schlagworte:
Communicable Disease Control - methods
Communicable Disease Control - statistics & numerical data
COVID-19
COVID-19 - epidemiology
COVID-19 - transmission
Disease transmission
Epidemiology
France
Genome, Viral
Humans
Pandemics
Paris - epidemiology
Physical Distancing
Quarantine
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
SARS-CoV-2 - isolation & purification
Severe acute respiratory syndrome coronavirus 2
Shelter in place
Viral Load
Virus Shedding
Wastewater - virology
Whole genome sequencing
France
Paris
Paris France
COVID-19
lockdown
SARS-CoV-2
quantification
waste water
ISSN:1560-7917, 1025-496X, 1560-7917
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Zusammenfassung:IntroductionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent of coronavirus disease (COVID-19). People infected with SARS-CoV-2 may exhibit no or mild non-specific symptoms; thus, they may contribute to silent circulation of the virus among humans. Since SARS-CoV-2 RNA can be detected in stool samples, monitoring SARS-CoV-2 RNA in waste water (WW) has been proposed as a complementary tool to investigate virus circulation in human populations.AimTo test if the quantification of SARS-CoV-2 genomes in WW correlates with the number of symptomatic or non-symptomatic carriers.MethodWe performed a time-course quantitative analysis of SARS-CoV-2 by RT-qPCR in raw WW samples collected from several major WW treatment plants in Greater Paris. The study period was 5 March to 23 April 2020, including the lockdown period in France (from 17 March).ResultsWe showed that the increase of genome units in raw WW accurately followed the increase of human COVID-19 cases observed at the regional level. Of note, the viral genome could be detected before the epidemic grew massively (around 8 March). Equally importantly, a marked decrease in the quantities of genome units was observed concomitantly with the reduction in the number of new COVID-19 cases, 29 days following the lockdown.ConclusionThis work suggests that a quantitative monitoring of SARS-CoV-2 genomes in WW could generate important additional information for improved monitoring of SARS-CoV-2 circulation at local or regional levels and emphasises the role of WW-based epidemiology.
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Correspondence: Laurent Moulin (laurent.moulin@eaudeparis.fr)
ISSN:1560-7917
1025-496X
1560-7917
DOI:10.2807/1560-7917.ES.2020.25.50.2000776