Differential cellular uptake and metabolism of curcuminoids in monocytes/macrophages: regulatory effects on lipid accumulation

We have previously shown that curcumin (CUR) may increase lipid accumulation in cultured human acute monocytic leukaemia cell line THP-1 monocytes/macrophages, but that tetrahydrocurcumin (THC), an in vivo metabolite of CUR, has no such effect. In the present study, we hypothesised that the differen...

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Veröffentlicht in:British journal of nutrition Jg. 112; H. 1; S. 8 - 14
Hauptverfasser: Nakagawa, Kiyotaka, Zingg, Jean-Marc, Kim, Sharon H., Thomas, Michael J., Dolnikowski, Gregory G., Azzi, Angelo, Miyazawa, Teruo, Meydani, Mohsen
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Cambridge Cambridge University Press 14.07.2014
Schlagworte:
Accumulation
bioactive properties
Biological and medical sciences
Biological Transport
Carcinogens - pharmacology
Cell Differentiation - drug effects
Cell Line
cell lines
Cellular biology
chromatography
Chromatography, High Pressure Liquid
curcumin
Curcumin - analogs & derivatives
Curcumin - chemistry
Curcumin - metabolism
Diarylheptanoids
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Glucuronides - chemistry
Glucuronides - metabolism
Humans
Kinetics
Leukemia
Lipid Metabolism
Lipids
macrophages
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Metabolism
Metabolites
monocytes
Monocytes - cytology
Monocytes - drug effects
Monocytes - metabolism
Nutrition
Phenols
sulfates
Sulfates - chemistry
Sulfates - metabolism
Tandem Mass Spectrometry
Tetradecanoylphorbol Acetate - analogs & derivatives
Tetradecanoylphorbol Acetate - pharmacology
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Monocytes
Monocyte
Nutrition
Curcuminoids
Lipids
Metabolism
Macrophages
Uptake
Macrophage
ISSN:0007-1145, 1475-2662, 1475-2662
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Zusammenfassung:We have previously shown that curcumin (CUR) may increase lipid accumulation in cultured human acute monocytic leukaemia cell line THP-1 monocytes/macrophages, but that tetrahydrocurcumin (THC), an in vivo metabolite of CUR, has no such effect. In the present study, we hypothesised that the different cellular uptake and/or metabolism of CUR and THC might be a possible explanation for the previously observed differences in their effects on lipid accumulation in THP-1 monocytes/macrophages. Chromatography with tandem MS revealed that CUR was readily taken up by THP-1 monocytes/macrophages and slowly metabolised to hexahydrocurcumin sulphate. By contrast, the uptake of THC was low. In parallel with CUR uptake, increased lipid uptake was observed in THP-1 macrophages but not with the uptake of THC or another CUR metabolite and structurally related compounds. From these results, it is possible to deduce that CUR and THC are taken up and metabolised differently in THP-1 cells, which determine their biological activity. The remarkable differential cellular uptake of CUR, relative to THC and other similar molecules, may imply that the CUR uptake into cells may occur via a transporter.
Bibliographie:ObjectType-Article-2
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ISSN:0007-1145
1475-2662
1475-2662
DOI:10.1017/S0007114514000567