Characterization and optimization of carbohydrate production from an indigenous microalga Chlorella vulgaris FSP-E

► An indigenous C. vulgaris FSP-E isolate exhibits high potential as sugar producer. ► Microalgal growth is improved by properly adjusting light intensity and inoculum size. ► Nitrogen starvation is very effective in promoting carbohydrate accumulation. ► The carbohydrate profile of the microalga is...

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Published in:Bioresource technology Vol. 135; pp. 157 - 165
Main Authors: Ho, Shih-Hsin, Huang, Shu-Wen, Chen, Chun-Yen, Hasunuma, Tomohisa, Kondo, Akihiko, Chang, Jo-Shu
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.05.2013
Subjects:
Batch Cell Culture Techniques
bioethanol
Biomass
biosynthesis
Biotechnology
Biotechnology - methods
Carbohydrate
carbohydrate content
Carbohydrates
Carbohydrates - biosynthesis
cell growth
Chlorella vulgaris
Chlorella vulgaris - growth & development
Chlorella vulgaris - isolation & purification
Chlorella vulgaris - metabolism
Chlorella vulgaris - radiation effects
Feedstock
feedstocks
Fermentation
Glucose
growth & development
inoculum
isolation & purification
Light
Light intensity
metabolism
methods
Microalgae
Microalgae - growth & development
Microalgae - isolation & purification
Microalgae - metabolism
Microalgae - radiation effects
nitrogen
Nitrogen - pharmacology
Nitrogen starvation
Optimization
pharmacology
Productivity
radiation effects
starvation
Strategy
Time Factors
Light intensity
Microalgae
Carbohydrate
Nitrogen starvation
Chlorella vulgaris
ISSN:0960-8524, 1873-2976, 1873-2976
Online Access:Get full text
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Summary:► An indigenous C. vulgaris FSP-E isolate exhibits high potential as sugar producer. ► Microalgal growth is improved by properly adjusting light intensity and inoculum size. ► Nitrogen starvation is very effective in promoting carbohydrate accumulation. ► The carbohydrate profile of the microalga is suitable for bioethanol fermentation. In this study, three indigenous microalgae isolates were examined for their ability to produce carbohydrates. Among them, Chlorella vulgaris FSP-E displayed relatively high cell growth rate and carbohydrate content. The carbohydrate productivity of C. vulgaris FSP-E was further improved by using engineering strategies. The results show that using an appropriate light intensity and inoculum size could effectively promote cell growth and carbohydrate productivity. Nitrogen starvation triggered the accumulation of carbohydrates in the microalga, achieving a carbohydrate content of 51.3% after 4-day starvation. Under the optimal conditions, the highest biomass and carbohydrate productivity were 1.437 and 0.631gL−1d−1, respectively. This performance is better than that reported in most related studies. Since glucose accounted for nearly 93% of the carbohydrates accumulated in C. vulgaris FSP-E, the microalga is an excellent feedstock for bioethanol fermentation.
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ISSN:0960-8524
1873-2976
1873-2976
DOI:10.1016/j.biortech.2012.10.100