Co-delivery of siRNAs and anti-cancer drugs using layered double hydroxide nanoparticles

In this research we employed layered double hydroxide nanoparticles (LDHs) to simultaneously deliver an anticancer drug 5-fluorouracil (5-FU) and Allstars Cell Death siRNA (CD-siRNA) for effective cancer treatment. The strategy takes advantage of the LDH anion exchange capacity to intercalate 5-FU i...

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Bibliographic Details
Published in:Biomaterials Vol. 35; no. 10; pp. 3331 - 3339
Main Authors: Li, Li, Gu, Wenyi, Chen, Jiezhong, Chen, Weiyu, Xu, Zhi P.
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01.03.2014
Subjects:
Advanced Basic Science
anion exchange capacity
Antineoplastic Agents - administration & dosage
Apoptosis
Blotting, Western
cell death
Cell Line, Tumor
Co-delivery system
cytotoxicity
Dentistry
drug resistance
Electrostatic assembly
fluorouracil
Humans
Hydroxides - chemistry
Layered double hydroxide
mitochondria
Nanoparticles
neoplasms
RNA, Small Interfering - administration & dosage
small interfering RNA
Co-delivery system
Layered double hydroxide
Electrostatic assembly
Apoptosis
ISSN:0142-9612, 1878-5905, 1878-5905
Online Access:Get full text
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Summary:In this research we employed layered double hydroxide nanoparticles (LDHs) to simultaneously deliver an anticancer drug 5-fluorouracil (5-FU) and Allstars Cell Death siRNA (CD-siRNA) for effective cancer treatment. The strategy takes advantage of the LDH anion exchange capacity to intercalate 5-FU into its interlayer spacing and load siRNA on the surface of LDH nanoparticles. LDH nanoparticles have been previously demonstrated as an effective cellular delivery system for 5-FU and siRNA separately in various investigations. More excitedly, the combination of CD-siRNA and anticancer drug 5-FU with the same LDH particles significantly enhanced cytotoxicity to three cancer cell lines, e.g. MCF-7, U2OS and HCT-116, compared to the single treatment with either CD-siRNA or 5-FU. This enhancement is probably a result of coordinate mitochondrial damage process. Thus, the strategy to co-deliver siRNA and an anticancer drug by LDHs has great potential to overcome the drug resistance and enhance cancer treatment.
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ISSN:0142-9612
1878-5905
1878-5905
DOI:10.1016/j.biomaterials.2013.12.095