Identification of differentially expressed genes in tobacco chewing-mediated oral cancer by differential display-polymerase chain reaction

Background  Identification of changes in gene expression that occur in oral squamous cell carcinoma (OSCC), after sufficient characterization, may yield novel molecular markers that may be useful in the diagnosis and disease management of oral cancer. Materials and methods  We used differential disp...

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Bibliographic Details
Published in:European journal of clinical investigation Vol. 37; no. 8; pp. 658 - 664
Main Authors: Nagpal, J. K., Das, B. R.
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.08.2007
Blackwell
Subjects:
Biological and medical sciences
Blotting, Northern - methods
Carcinoma, Squamous Cell - chemically induced
Carcinoma, Squamous Cell - genetics
CDK6
Cell Line, Tumor
DD-PCR
DEK
Developing Countries
Gene Expression Regulation, Neoplastic - genetics
General aspects
Human immunodeficiency virus
Humans
Medical sciences
Mouth Neoplasms - chemically induced
Mouth Neoplasms - genetics
oral cancer
Polymerase Chain Reaction - methods
sorcin
tobacco chewing
Tobacco, Smokeless - adverse effects
Tobacco, tobacco smoking
Toxicology
tobacco chewing
Sorcin
Oral cancer
Stomatology
Tobacco smoking
Identification
Malignant tumor
Chewing
DEK
Gene expression
DD-PCR
Medicine
Polymerase chain reaction
Oral cavity
Tobacco
CDK6
Oral cavity disease
Molecular biology
ISSN:0014-2972, 1365-2362
Online Access:Get full text
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Summary:Background  Identification of changes in gene expression that occur in oral squamous cell carcinoma (OSCC), after sufficient characterization, may yield novel molecular markers that may be useful in the diagnosis and disease management of oral cancer. Materials and methods  We used differential display–polymerase chain reaction (DD‐PCR) to study critically the global gene expression profile of the oral tumour versus normal epithelium. The differential expression of fished out cDNA were confirmed by Northern blot and reverse transcription‐PCR. The differentially expressed cDNA were cloned, sequenced and matched for homology in the GenBank database. Results  We identified 13 cDNA that showed differential expression. Out of these we selected four cDNA showing consistent reproducibility. One of the cDNA expressed exclusively in tumour had a homology to DEK, a putative oncogene, and is linked to leukaemia, various cancers, HIV infection and several autoimmune disorders. Another cDNA expressed only in tumour had homology to sorcin protein. Sorcin is a 22‐kDa calcium‐binding protein and is associated with drug resistance in various cell lines. Apparently, sorcin expression might be responsible for drug resistance of OSCC and poor prognosis. Another cDNA showing 10 times overexpression in cheek tumour as compared to normal had homology to CDK6 gene. Hence, it seems from our results that CDK6 is dysregulated during oral carcinogenesis. The fourth cDNA was overexpressed in normal as compared to cheek tumour, but did not show any match in BLAST search. Conclusions  We conclude that there is an enormous significance of these differentially expressed cDNA in oral cancer progression as they can serve as cancer markers to be used for diagnosis and therapeutic intervention.
Bibliography:istex:581FC29DEC56088C38202A13925DB9A2EABF5182
ArticleID:ECI1841
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Molecular Oncology and Medical Biotechnology Division, Institute of Life Sciences, Nalco Square, Chandrasekharpur, Bhubaneswar–751 023, India (J. K. Nagpal); Research & Development Division, SRL‐Ranbaxy Limited, Clinical Reference Laboratories, Mumbai‐400 093, India (B. R. Das).
Present address: Department of Otolaryngology & Head and Neck Surgery, Johns Hopkins University, 1550 Orleans Street, Baltimore, MD21231, USA.
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content type line 23
ISSN:0014-2972
1365-2362
DOI:10.1111/j.1365-2362.2007.01841.x