Exosome Processing and Characterization Approaches for Research and Technology Development

Exosomes are extracellular vesicles that share components of their parent cells and are attractive in biotechnology and biomedical research as potential disease biomarkers as well as therapeutic agents. Crucial to realizing this potential is the ability to manufacture high‐quality exosomes; however,...

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Bibliographic Details
Published in:Advanced science Vol. 9; no. 15; pp. e2103222 - n/a
Main Authors: Lai, James J., Chau, Zoe L., Chen, Sheng‐You, Hill, John J., Korpany, Katalin V., Liang, Nai‐Wen, Lin, Li‐Han, Lin, Yi‐Hsuan, Liu, Joanne K., Liu, Yu‐Chung, Lunde, Ruby, Shen, Wei‐Ting
Format: Journal Article
Language:English
Published: Germany John Wiley & Sons, Inc 01.05.2022
John Wiley and Sons Inc
Wiley
Subjects:
Acids
analytical characterizations
Apoptosis
Biomarkers
Biosynthesis
Breast cancer
exosome clinical trials
exosomes
Exosomes - chemistry
Exosomes - metabolism
Extracellular Vesicles
Good Manufacturing Practices
Industrial Development
Infectious diseases
isolation processes
Kinases
Lipids
Membranes
MicroRNAs
MISEV2018 guidelines
Mitochondrial DNA
Plasma
Proteins
Proteins - metabolism
Review
Reviews
extracellular vesicles
exosomes
MISEV2018 guidelines
analytical characterizations
Good Manufacturing Practices
exosome clinical trials
isolation processes
ISSN:2198-3844, 2198-3844
Online Access:Get full text
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Summary:Exosomes are extracellular vesicles that share components of their parent cells and are attractive in biotechnology and biomedical research as potential disease biomarkers as well as therapeutic agents. Crucial to realizing this potential is the ability to manufacture high‐quality exosomes; however, unlike biologics such as proteins, exosomes lack standardized Good Manufacturing Practices for their processing and characterization. Furthermore, there is a lack of well‐characterized reference exosome materials to aid in selection of methods for exosome isolation, purification, and analysis. This review informs exosome research and technology development by comparing exosome processing and characterization methods and recommending exosome workflows. This review also provides a detailed introduction to exosomes, including their physical and chemical properties, roles in normal biological processes and in disease progression, and summarizes some of the on‐going clinical trials. The capability to manufacture high quality exosomes is essential for fully realizing their potential as disease biomarkers as well as therapeutic agents. This review informs exosome research and technology development by comparing exosome processing and characterization methods and recommending exosome workflows. The figure shows relative yield, purity, and throughput of different exosome isolation techniques.
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ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202103222