A role of salivary carbonic anhydrase VI in dental plaque

Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy sub...

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Vydáno v:	Archives of Oral Biology Ročník 51; číslo 2; s. 117 - 122
Hlavní autoři:	Kimoto, Masaya, Kishino, Mitsunobu, Yura, Yoshiaki, Ogawa, Yuzo
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Ltd 01.02.2006 Elsevier BV
Témata:	Acetazolamide Acetazolamide - pharmacology Adult Blotting, Western Carbonic Anhydrase Inhibitors Carbonic Anhydrase Inhibitors - pharmacology Carbonic anhydrase VI Carbonic Anhydrases Carbonic Anhydrases - physiology Dental Plaque Dental Plaque - enzymology Dental Plaque - metabolism Enzyme histochemistry Female Humans Hydrogen-Ion Concentration Immunoenzyme Techniques Immunohistochemistry Male Protein Subunits Protein Subunits - antagonists & inhibitors Protein Subunits - physiology Saliva Saliva - enzymology Sucrose Western analysis Immunohistochemistry Western analysis Carbonic anhydrase VI Dental plaque pH Saliva Enzyme histochemistry
ISSN:	0003-9969, 1879-1506
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Abstract	Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10 −5 M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis. Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II. The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva.
AbstractList	Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10 −5 M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis. Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II. The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva. Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO2 +H2O<-->H+ +HCO3-. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10(-5)M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis. Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II. The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva. Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO2 +H2O<-->H+ +HCO3-. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva.OBJECTIVECarbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO2 +H2O<-->H+ +HCO3-. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva.Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10(-5)M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis.DESIGNNine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10(-5)M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis.Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II.RESULTSThough sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II.The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva.CONCLUSIONSThe results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva.
Author	Ogawa, Yuzo Yura, Yoshiaki Kishino, Mitsunobu Kimoto, Masaya
Author_xml	– sequence: 1 givenname: Masaya surname: Kimoto fullname: Kimoto, Masaya organization: Department of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan – sequence: 2 givenname: Mitsunobu surname: Kishino fullname: Kishino, Mitsunobu organization: Department of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan – sequence: 3 givenname: Yoshiaki surname: Yura fullname: Yura, Yoshiaki organization: Department of Oral and Maxillofacial Surgery II, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan – sequence: 4 givenname: Yuzo surname: Ogawa fullname: Ogawa, Yuzo email: ogawa@dent.osaka-u.ac.jp organization: Department of Oral Pathology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
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Snippet	Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to... Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO2 +H2O<-->H+ +HCO3-. CA VI has been thought to provide...
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SubjectTerms	Acetazolamide Acetazolamide - pharmacology Adult Blotting, Western Carbonic Anhydrase Inhibitors Carbonic Anhydrase Inhibitors - pharmacology Carbonic anhydrase VI Carbonic Anhydrases Carbonic Anhydrases - physiology Dental Plaque Dental Plaque - enzymology Dental Plaque - metabolism Enzyme histochemistry Female Humans Hydrogen-Ion Concentration Immunoenzyme Techniques Immunohistochemistry Male Protein Subunits Protein Subunits - antagonists & inhibitors Protein Subunits - physiology Saliva Saliva - enzymology Sucrose Western analysis
Title	A role of salivary carbonic anhydrase VI in dental plaque
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