A role of salivary carbonic anhydrase VI in dental plaque

Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy sub...

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Published in:Archives of Oral Biology Vol. 51; no. 2; pp. 117 - 122
Main Authors: Kimoto, Masaya, Kishino, Mitsunobu, Yura, Yoshiaki, Ogawa, Yuzo
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.02.2006
Elsevier BV
Subjects:
Acetazolamide
Acetazolamide - pharmacology
Adult
Blotting, Western
Carbonic Anhydrase Inhibitors
Carbonic Anhydrase Inhibitors - pharmacology
Carbonic anhydrase VI
Carbonic Anhydrases
Carbonic Anhydrases - physiology
Dental Plaque
Dental Plaque - enzymology
Dental Plaque - metabolism
Enzyme histochemistry
Female
Humans
Hydrogen-Ion Concentration
Immunoenzyme Techniques
Immunohistochemistry
Male
Protein Subunits
Protein Subunits - antagonists & inhibitors
Protein Subunits - physiology
Saliva
Saliva - enzymology
Sucrose
Western analysis
Immunohistochemistry
Western analysis
Carbonic anhydrase VI
Dental plaque
pH
Saliva
Enzyme histochemistry
ISSN:0003-9969, 1879-1506
Online Access:Get full text
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Summary:Carbonic anhydrase (CA) VI is a unique secreted isozyme of CA, which catalyzes the reversible reaction CO 2 + H 2O ↔ H + + HCO 3 −. CA VI has been thought to provide a greater buffering capacity to fluids into which it is secreted. This study was performed to confirm this in saliva. Nine healthy subjects participated in the study. The pH of the dental plaque from each subject was monitored after a mouth rinse with 10% sucrose with or without 10 −5 M acetazolamide, a specific inhibitor of CA. Also CA was examined in plaque by enzyme histochemistry, immunohistochemistry and Western blot analysis. Though sucrose and sucrose plus inhibitor yielded Stephan curves with a similar temporal pattern, the pH values of the latter were significantly lower than those of the former. Plaque exhibited CA activity by enzyme histochemistry. Immunohistochemistry and Western analysis demonstrated that the activity was due to CA VI but not to CA I or CA II. The results indicate that CA VI in saliva penetrates plaque and facilitates acid neutralization by salivary bicarbonate. Therefore, CA VI may be considered an anti-caries protein in saliva.
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ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2005.04.007