Pathogens Detected in Questing Ixodes ricinus Ticks in a Mountainous Area in Greece

Ixodes ricinus ticks are vectors of a plethora of pathogens. The purpose of this study was to screen 398 I. ricinus ticks for a variety of pathogens. Following the pooling, homogenization, and extraction of total nucleic acids, a real-time PCR was applied for the detection of a panel of tick-borne p...

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Vydané v:	Pathogens (Basel) Ročník 13; číslo 6; s. 449
Hlavní autori:	Tsioka, Katerina, Saratsis, Anastasios, Pappa, Styliani, Papa, Anna
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Switzerland MDPI AG 25.05.2024 MDPI
Predmet:	Anaplasma phagocytophilum Anaplasma phagocytophilum - genetics Anaplasma phagocytophilum - isolation & purification Animals Arachnids Borrelia Borrelia - genetics Borrelia - isolation & purification Borrelia garinii Borrelia valaisiana Clustering Encephalitis genus Goats Greece homogenization Ixodes - microbiology Ixodes - virology Ixodes ricinus monitoring Mountain regions Mountainous areas mountains Nucleic acids Orthonairovirus Pathogens quantitative polymerase chain reaction Real time Real-Time Polymerase Chain Reaction Rickettsia Rickettsia - genetics Rickettsia - isolation & purification species Surveillance tick-borne pathogens Ticks Vectors Vegetation Viruses Greece Germany Greece Borrelia Ixodes ricinus Rickettsia tick-borne pathogens
ISSN:	2076-0817, 2076-0817
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Shrnutí:	Ixodes ricinus ticks are vectors of a plethora of pathogens. The purpose of this study was to screen 398 I. ricinus ticks for a variety of pathogens. Following the pooling, homogenization, and extraction of total nucleic acids, a real-time PCR was applied for the detection of a panel of tick-borne pathogens, while additional conventional PCRs combined with Sanger sequencing were applied for the detection of viruses and typing of Rickettsia and Borrelia species. At least one pathogen was detected in 60 of the 80 (75%) tick pools. Rickettsia spp. predominated, as it was detected in 63.75% of the pools (51/80; MIR 12.81%), followed by Borrelia spp. (35 pools (45%); MIR 8.79%), while Anaplasma phagocytophilum was detected in 2 pools (2.5%, MIR 0.5%). The ticks of six Rickettsia-positive pools were tested individually (from stored half-ticks); all sequences were identical to those of R. monacensis. Similarly, the ticks of six Borrelia-positive pools were tested individually, and it was shown that four belonged to the genospecies Borrelia garinii and two to Borrelia valaisiana. Phleboviruses were detected in 3 pools (3.75%; MIR 0.75%), with sequences clustering in the Ixovirus genus, while nairoviruses were detected in 7 pools (8.75%; MIR 1.76%), with one sequence clustering in the Orthonairovirus genus, and six clustering in the Norwavirus genus. Although a small number of ticks from only one area in Greece were tested, a variety of pathogens together with recently identified viruses were detected, prompting further studies in ticks and surveillance studies in humans.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work.
ISSN:	2076-0817 2076-0817
DOI:	10.3390/pathogens13060449