Human skin penetration of hyaluronic acid of different molecular weights as probed by Raman spectroscopy

Background Topical delivery of molecules into the human skin is one of the main issues in dermatology and cosmetology. Several techniques were developed to study molecules penetration into the human skin. Although widely accepted, the conventional methods such as Franz diffusion cells are unable to...

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Bibliographic Details
Published in:Skin research and technology Vol. 22; no. 1; pp. 55 - 62
Main Authors: Essendoubi, M., Gobinet, C., Reynaud, R., Angiboust, J. F., Manfait, M., Piot, O.
Format: Journal Article
Language:English
Published: England Blackwell Publishing Ltd 01.02.2016
John Wiley & Sons, Inc
Subjects:
Administration, Cutaneous
Adult
Female
Humans
Hyaluronic Acid - administration & dosage
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacokinetics
hyaluronic acid of different molecular weight
In Vitro Techniques
localization
Molecular Weight
penetration/permeation
Raman spectroscopy
skin
Skin - chemistry
Skin - metabolism
Skin Absorption - physiology
Spectrum Analysis, Raman - methods
Structure-Activity Relationship
localization
penetration/permeation
skin
hyaluronic acid of different molecular weight
Raman spectroscopy
ISSN:0909-752X, 1600-0846, 1600-0846
Online Access:Get full text
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Summary:Background Topical delivery of molecules into the human skin is one of the main issues in dermatology and cosmetology. Several techniques were developed to study molecules penetration into the human skin. Although widely accepted, the conventional methods such as Franz diffusion cells are unable to provide the accurate localization of actives in the skin layers. A different approach based on Raman spectroscopy has been proposed to follow‐up the permeation of actives. It presents a high molecular specificity to distinguish exogenous molecules from skin constituents. Methods Raman micro‐imaging was applied to monitor the skin penetration of hyaluronic acids (HA) of different molecular weights. The first step, was the spectral characterization of these HA. After, we have determined spectral features of HA by which they can be detected in the skin. In the second part, transverse skin sections were realized and spectral images were recorded. Results Our results show a difference of skin permeation of the three HA. Indeed, HA with low molecular weight (20–300 kDa) passes through the stratum corneum in contrast of the impermeability of high molecular weight HA (1000–1400 kDa). Conclusion Raman spectroscopy represents an analytical, non‐destructive, and dynamic method to evaluate the permeation of actives in the skin layers.
Bibliography:istex:FF11033C9AFD14E4DD811070E832037008F97F3D
ark:/67375/WNG-W57QGRFS-R
ArticleID:SRT12228
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0909-752X
1600-0846
1600-0846
DOI:10.1111/srt.12228