Interleukin-1β Level Is Increased in Vitreous of Patients with Neovascular Age-Related Macular Degeneration (nAMD) and Polypoidal Choroidal Vasculopathy (PCV)

To examine the expression of pro-interleukin-1β (pro-IL-1β) and interleukin-1β (IL-1β) in the vitreous body of patients with neovascular age-related macular degeneration(nAMD), polypoidal choroidal vasculopathy (PCV), proliferative diabetic retinopathy (PDR), retinal vein occlusion (RVO) or Eales�...

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Published in:PloS one Vol. 10; no. 5; p. e0125150
Main Authors: Zhao, Min, Bai, Yujing, Xie, Wankun, Shi, Xuan, Li, Fangting, Yang, Fei, Sun, Yaoyao, Huang, Lvzhen, Li, Xiaoxin
Format: Journal Article
Language:English
Published: United States Public Library of Science 15.05.2015
Public Library of Science (PLoS)
Subjects:
Active control
Adapter proteins
Age
Age related diseases
Aged
Choroid Diseases - blood
Choroid Diseases - metabolism
Choroidal Neovascularization - blood
Choroidal Neovascularization - metabolism
Cytokines
Diabetes
Diabetes mellitus
Diabetic retinopathy
Education
Enzyme-linked immunosorbent assay
Enzymes
Female
Hemorrhage
Hospitals
Humans
IL-1β
Inflammation
Interleukin-1beta - blood
Interleukin-1beta - metabolism
Interleukins
Laboratories
Macular degeneration
Macular Degeneration - blood
Macular Degeneration - metabolism
Male
Middle Aged
Occlusion
Pathogenesis
Patients
Pattern recognition
Peripheral Vascular Diseases - blood
Peripheral Vascular Diseases - metabolism
Polyps - blood
Polyps - metabolism
Prospective Studies
Protein folding
Proteins
Retina
Retinal degeneration
Retinopathy
Statistical analysis
Statistical methods
Surgery
Tumor necrosis factor-TNF
Vascular diseases
Vascularization
Vitreous Body - metabolism
ISSN:1932-6203, 1932-6203
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Summary:To examine the expression of pro-interleukin-1β (pro-IL-1β) and interleukin-1β (IL-1β) in the vitreous body of patients with neovascular age-related macular degeneration(nAMD), polypoidal choroidal vasculopathy (PCV), proliferative diabetic retinopathy (PDR), retinal vein occlusion (RVO) or Eales' disease to further elucidate the role of IL-1β and inflammation in the pathogenesis of neovascular retinal disease. Prospective clinical laboratory investigation study. All patients enrolled had vitreous hemorrhage due to nAMD, PCV, PDR, RVO or Eales' disease that required vitrectomy. Patients were excluded for any history of active intraocular inflammation, or other ophthalmic surgery besides vitrectomy. Control samples were obtained from patients with idiopathic macular epiretinal membrane. A total of fifty vitreous samples were collected from patient during vitrectomy. Pro-IL-1β and IL-1β expression were measured by enzyme-linked immunosorbent assay (ELISA). Results were analyzed statistically using nonparametric tests. Expression of pro-IL-1β protein was increased by 2.83-fold and 9.19-fold in PCV and nAMD vitreous samples relative to control, respectively. Expression of IL-β protein was increased by 10-fold and 4.83-fold in PCV and nAMD vitreous samples relative to control, respectively. Our results demonstrate that expression of pro-IL-1β and IL-1β proteins is higher in PCV and nAMD. The roles of pro-IL-1β and IL-1β as inflammatory mediators in the development of PCV and nAMD may be associated with photoreceptor degeneration and neovascularization which necessitates further study.
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: XL XS. Performed the experiments: MZ YB WX. Analyzed the data: YS LH. Contributed reagents/materials/analysis tools: FL FY. Wrote the paper: MZ YB WX.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0125150