Interleukin-1β Level Is Increased in Vitreous of Patients with Neovascular Age-Related Macular Degeneration (nAMD) and Polypoidal Choroidal Vasculopathy (PCV)

To examine the expression of pro-interleukin-1β (pro-IL-1β) and interleukin-1β (IL-1β) in the vitreous body of patients with neovascular age-related macular degeneration(nAMD), polypoidal choroidal vasculopathy (PCV), proliferative diabetic retinopathy (PDR), retinal vein occlusion (RVO) or Eales�...

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Vydáno v:	PloS one Ročník 10; číslo 5; s. e0125150
Hlavní autoři:	Zhao, Min, Bai, Yujing, Xie, Wankun, Shi, Xuan, Li, Fangting, Yang, Fei, Sun, Yaoyao, Huang, Lvzhen, Li, Xiaoxin
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States Public Library of Science 15.05.2015 Public Library of Science (PLoS)
Témata:	Active control Adapter proteins Age Age related diseases Aged Choroid Diseases - blood Choroid Diseases - metabolism Choroidal Neovascularization - blood Choroidal Neovascularization - metabolism Cytokines Diabetes Diabetes mellitus Diabetic retinopathy Education Enzyme-linked immunosorbent assay Enzymes Female Hemorrhage Hospitals Humans IL-1β Inflammation Interleukin-1beta - blood Interleukin-1beta - metabolism Interleukins Laboratories Macular degeneration Macular Degeneration - blood Macular Degeneration - metabolism Male Middle Aged Occlusion Pathogenesis Patients Pattern recognition Peripheral Vascular Diseases - blood Peripheral Vascular Diseases - metabolism Polyps - blood Polyps - metabolism Prospective Studies Protein folding Proteins Retina Retinal degeneration Retinopathy Statistical analysis Statistical methods Surgery Tumor necrosis factor-TNF Vascular diseases Vascularization Vitreous Body - metabolism
ISSN:	1932-6203, 1932-6203
On-line přístup:	Získat plný text
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Popis
Shrnutí:	To examine the expression of pro-interleukin-1β (pro-IL-1β) and interleukin-1β (IL-1β) in the vitreous body of patients with neovascular age-related macular degeneration(nAMD), polypoidal choroidal vasculopathy (PCV), proliferative diabetic retinopathy (PDR), retinal vein occlusion (RVO) or Eales' disease to further elucidate the role of IL-1β and inflammation in the pathogenesis of neovascular retinal disease. Prospective clinical laboratory investigation study. All patients enrolled had vitreous hemorrhage due to nAMD, PCV, PDR, RVO or Eales' disease that required vitrectomy. Patients were excluded for any history of active intraocular inflammation, or other ophthalmic surgery besides vitrectomy. Control samples were obtained from patients with idiopathic macular epiretinal membrane. A total of fifty vitreous samples were collected from patient during vitrectomy. Pro-IL-1β and IL-1β expression were measured by enzyme-linked immunosorbent assay (ELISA). Results were analyzed statistically using nonparametric tests. Expression of pro-IL-1β protein was increased by 2.83-fold and 9.19-fold in PCV and nAMD vitreous samples relative to control, respectively. Expression of IL-β protein was increased by 10-fold and 4.83-fold in PCV and nAMD vitreous samples relative to control, respectively. Our results demonstrate that expression of pro-IL-1β and IL-1β proteins is higher in PCV and nAMD. The roles of pro-IL-1β and IL-1β as inflammatory mediators in the development of PCV and nAMD may be associated with photoreceptor degeneration and neovascularization which necessitates further study.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: XL XS. Performed the experiments: MZ YB WX. Analyzed the data: YS LH. Contributed reagents/materials/analysis tools: FL FY. Wrote the paper: MZ YB WX.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0125150