Evolution of multiple, mutually orthogonal prolyl-tRNA synthetase/tRNA pairs for unnatural amino acid mutagenesis in Escherichia coli

The site-specific incorporation of unnatural amino acids (UAAs) into proteins in living cells relies on an engineered tRNA/aminoacyl-tRNA synthetase (tRNA/aaRS) pair, orthogonal to the host cell, to deliver the UAA of choice in response to a unique nonsense or frameshift codon. Here we report the ge...

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Vydané v:	Proceedings of the National Academy of Sciences - PNAS Ročník 109; číslo 37; s. 14841
Hlavní autori:	Chatterjee, Abhishek, Xiao, Han, Schultz, Peter G
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 11.09.2012
Predmet:	Amino Acids - metabolism Amino Acyl-tRNA Synthetases - genetics Archaeoglobus fulgidus - genetics Cloning, Molecular Escherichia coli Evolution, Molecular Gene Library Mutagenesis, Site-Directed - methods Plasmids - genetics Protein Engineering - methods Pyrococcus horikoshii - genetics RNA, Transfer - genetics RNA, Transfer - metabolism
ISSN:	1091-6490, 1091-6490
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