Neurogenesis and progenitor cell distribution in the subgranular zone and subventricular zone of the adult sheep brain

•We studied adult sheep neurogenic niches after administering BrdU.•Progenitor cell distribution in the sheep subventricular zone is similar to humans.•The sheep subventricular zone has far more progenitors than the hippocampus.•The overall structure of the sheep subventricular zone is similar to hu...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience Vol. 244; pp. 173 - 187
Main Authors: Low, V.F., Faull, R.L.M., Bennet, L., Gunn, A.J., Curtis, M.A.
Format: Journal Article
Language:English
Published: Amsterdam Elsevier Ltd 06.08.2013
Elsevier
Subjects:
Age Factors
animal model
Animals
Biological and medical sciences
Brain - cytology
Brain - growth & development
brain plasticity
Cell Proliferation
Female
Fundamental and applied biological sciences. Psychology
germinal niche
Neurogenesis
Neurology
Sheep
Stem Cells - cytology
Stem Cells - physiology
transgenic sheep
Vertebrates: nervous system and sense organs
PBS
DG
PCNA
DAB
TBS
transgenic sheep
EL
SGZ
CN
GFAP
GCL
TBS-T
germinal niche
NeuN
SVZ
neurogenesis
RMS
HD
animal model
BrdU
brain plasticity
caudate nucleus
neuronal nuclear antigen
5-bromo-2′-deoxyuridine
phosphate-buffered saline
tris-buffered saline with Triton X-100
Huntington’s disease
rostral migratory stream
subventricular zone
3,3-diaminobenzidine
granule cell layer
subgranular zone
proliferating cell nuclear antigen
dentate gyrus
tris-buffered saline
ependymal layer
glial fibrillary acidic protein
Animal model
Central nervous system
Precursor cell
Transgenic animal
Neurogenesis
Encephalon
Vertebrata
Mammalia
Distribution
Plasticity
Sheep
Artiodactyla
Ungulata
Progenitor cell
ISSN:0306-4522, 1873-7544, 1873-7544
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•We studied adult sheep neurogenic niches after administering BrdU.•Progenitor cell distribution in the sheep subventricular zone is similar to humans.•The sheep subventricular zone has far more progenitors than the hippocampus.•The overall structure of the sheep subventricular zone is similar to humans. Progenitor cell proliferation is ubiquitous in the subventricular zone (SVZ) and subgranular zone (SGZ) of adult mammalian brains, however, the abundance and distribution of proliferation are surprisingly heterogeneous between species. In rodents, proliferation is high in both the SVZ and SGZ, while in humans proliferation is prominent in the SVZ but limited in the SGZ. To accurately study proliferation and how it changes in human disease, we should focus on animals in which the patterns of proliferation are consistent with the human brain. In this study, we characterized the neurogenic niches of the adult sheep, an animal model with a longer lifespan than rodents and a highly gyrencephalic brain, using 5-bromo-2′-deoxyuridine (BrdU) as a mitotic marker and neuronal nuclear antigen to identify neuronal lineage cells. Our study demonstrates that the sheep SVZ is organized into the same distinct layers that are comparable to what has been described in humans. The rate of maturation of new neurons was slower in sheep than in previous reports in rodents, with only 20% of BrdU-positive cells showing neuronal phenotype after 4 months survival following BrdU administration. Most importantly, as in the human, there was much greater proliferation in the sheep SVZ than in the SGZ. These results suggest that the sheep is a better basis for comparisons with human SVZ and SGZ neurogenesis than rodents.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0306-4522
1873-7544
1873-7544
DOI:10.1016/j.neuroscience.2013.04.006