Spatial transcriptomics in embryonic mouse diaphragm muscle reveals regional gradients and subdomains of developmental gene expression

The murine embryonic diaphragm is a primary model for studying myogenesis and neuro-muscular synaptogenesis, both representing processes regulated by spatially organized genetic programs of myonuclei located in distinct myodomains. However, a spatial gene expression pattern of embryonic mouse diaphr...

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Vydáno v:iScience Ročník 27; číslo 6; s. 110018
Hlavní autoři: Kaplan, Mehmet Mahsum, Zeidler, Maximilian, Knapp, Annabella, Hölzl, Martina, Kress, Michaela, Fritsch, Helga, Krogsdam, Anne, Flucher, Bernhard E.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Elsevier Inc 21.06.2024
Elsevier
Témata:
Developmental biology
Model organism
Molecular physiology
Transcriptomics
Developmental biology
Transcriptomics
Model organism
Molecular physiology
ISSN:2589-0042, 2589-0042
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Shrnutí:The murine embryonic diaphragm is a primary model for studying myogenesis and neuro-muscular synaptogenesis, both representing processes regulated by spatially organized genetic programs of myonuclei located in distinct myodomains. However, a spatial gene expression pattern of embryonic mouse diaphragm has not been reported. Here, we provide spatially resolved gene expression data for horizontally sectioned embryonic mouse diaphragms at embryonic days E14.5 and E18.5. These data reveal gene signatures for specific muscle regions with distinct maturity and fiber type composition, as well as for a central neuromuscular junction (NMJ) and a peripheral myotendinous junction (MTJ) compartment. Comparing spatial expression patterns of wild-type mice with those of transgenic mice lacking either the skeletal muscle calcium channel CaV1.1 or β-catenin, reveals curtailed muscle development and dysregulated expression of genes potentially involved in NMJ formation. Altogether, these datasets provide a powerful resource for further studies of muscle development and NMJ formation in the mouse. [Display omitted] •Spatial atlas for gene expression in embryonic and fetal mouse diaphragm•Documentation of novel gene expression spatially correlated with Chrna1•Spatial alterations of NMJ genes in mice lacking CaV1.1 or muscle β-catenin•Spatial aspects of diaphragm muscle development controlled by β-catenin Molecular physiology; Developmental biology; Transcriptomics; Model organism
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Present address: Omiqa Bioinformatics, Berlin, Germany
Present address: Department of Developmental Biology, Institute of Experimental Medicine of the Czech Academy of Sciences, 14220 Prague, Czechia
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ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2024.110018