The diversity of Rab GTPases in Entamoeba histolytica

Rab proteins are ubiquitous small GTP-binding proteins that form a highly conserved family and regulate vesicular trafficking. Recent completion of the genome of the enteric protozoan parasite Entamoeba histolytica enabled us to identify an extremely large number (>90) of putative Rab genes. Mult...

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Veröffentlicht in:Experimental parasitology Jg. 110; H. 3; S. 244 - 252
Hauptverfasser: Saito-Nakano, Yumiko, Loftus, Brendan J., Hall, Neil, Nozaki, Tomoyoshi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: San Diego, CA Elsevier Inc 01.07.2005
Elsevier
Schlagworte:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
deoxyribonucleic acid
DNA
EhRab
Entamoeba histolytica
Entamoeba histolytica - classification
Entamoeba histolytica - enzymology
Entamoeba histolytica - genetics
Entamoeba histolytica Rab
Fundamental and applied biological sciences. Psychology
Genetic Variation
GTP
guanosine 5′-triphosphate
Humans
Life cycle. Host-agent relationship. Pathogenesis
Membrane traffic
Molecular Sequence Data
Phylogeny
Protozoa
rab GTP-Binding Proteins - chemistry
rab GTP-Binding Proteins - classification
rab GTP-Binding Proteins - genetics
Rab GTPase
Sequence Alignment
Sequence Homology, Amino Acid
Membrane traffic
Rab GTPase
GTP
DNA
Entamoeba histolytica
Phylogeny
EhRab
Protozoa
Lobosea
Parasite
Phytogeny
ISSN:0014-4894, 1090-2449
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Zusammenfassung:Rab proteins are ubiquitous small GTP-binding proteins that form a highly conserved family and regulate vesicular trafficking. Recent completion of the genome of the enteric protozoan parasite Entamoeba histolytica enabled us to identify an extremely large number (>90) of putative Rab genes. Multiple alignment and phylogenic analysis of amebic, human, and yeast Rab showed that only 22 amebic Rab proteins including EhRab1, EhRab2, EhRab5, EhRab7, EhRab8, EhRab11, and EhRab21 showed significant similarity to Rab from other organisms. The 69 remaining amebic Rab proteins showed only moderate similarity (<40% identity) to Rab proteins from other organisms. Approximately one-third of Rab proteins including Rab7, Rab11, and RabC form 15 subfamilies, which contain up to nine isoforms. Approximately 70% of amebic Rab genes contain single or multiple introns, and this proportion is significantly higher than that of common genes in this organism. Twenty-five Rabs possess an atypical carboxyl terminus such as CXXX, XCXX, XXCX, XXXC, and no cysteine. We propose annotation of amebic Rab genes and discuss biological significance of this extraordinary diversity of EhRab proteins in this organism.
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ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2005.02.021