Nanozyme-strip for rapid local diagnosis of Ebola

Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, b...

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Bibliographic Details
Published in:Biosensors & bioelectronics Vol. 74; pp. 134 - 141
Main Authors: Duan, Demin, Fan, Kelong, Zhang, Dexi, Tan, Shuguang, Liang, Mifang, Liu, Yang, Zhang, Jianlin, Zhang, Panhe, Liu, Wei, Qiu, Xiangguo, Kobinger, Gary P., Fu Gao, George, Yan, Xiyun
Format: Journal Article
Language:English
Published: England Elsevier B.V 15.12.2015
Subjects:
biosensors
Biotechnology
Bunyavirus
Diagnosis
Ebola detection
Ebola virus
Ebolavirus
Ebolavirus - isolation & purification
ELISA
enzyme-linked immunosorbent assay
Equipment Design
glycoproteins
Glycoproteins - isolation & purification
Hemorrhagic Fever, Ebola - diagnosis
Hemorrhagic Fever, Ebola - virology
Humans
immunoaffinity chromatography
Immunochromatographic strip
Immunochromatography - instrumentation
magnetite
Magnetite Nanoparticles - chemistry
nanoparticles
Nanostructure
Nanozyme
Orthobunyavirus
Reagent Strips - analysis
screening
Sensitivity and Specificity
Strip
Vaccines
Viral Proteins - isolation & purification
Western Africa
Western Africa
Immunochromatographic strip
Nanozyme
Ebola detection
ISSN:0956-5663, 1873-4235
Online Access:Get full text
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Summary:Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. •We developed a Nanozyme-strip method, which is 100 times more sensitive than the colloidal gold strip.•The first report of introducing Fe3O4 nanozyme into immunochromatographic strip.•The novel method could be used as a valuable tool for diagnosis of Ebola and other infectious disease.
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ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2015.05.025