Nanozyme-strip for rapid local diagnosis of Ebola

Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, b...

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Vydáno v:	Biosensors & bioelectronics Ročník 74; s. 134 - 141
Hlavní autoři:	Duan, Demin, Fan, Kelong, Zhang, Dexi, Tan, Shuguang, Liang, Mifang, Liu, Yang, Zhang, Jianlin, Zhang, Panhe, Liu, Wei, Qiu, Xiangguo, Kobinger, Gary P., Fu Gao, George, Yan, Xiyun
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier B.V 15.12.2015
Témata:	biosensors Biotechnology Bunyavirus Diagnosis Ebola detection Ebola virus Ebolavirus Ebolavirus - isolation & purification ELISA enzyme-linked immunosorbent assay Equipment Design glycoproteins Glycoproteins - isolation & purification Hemorrhagic Fever, Ebola - diagnosis Hemorrhagic Fever, Ebola - virology Humans immunoaffinity chromatography Immunochromatographic strip Immunochromatography - instrumentation magnetite Magnetite Nanoparticles - chemistry nanoparticles Nanostructure Nanozyme Orthobunyavirus Reagent Strips - analysis screening Sensitivity and Specificity Strip Vaccines Viral Proteins - isolation & purification Western Africa Western Africa Immunochromatographic strip Nanozyme Ebola detection
ISSN:	0956-5663, 1873-4235
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. •We developed a Nanozyme-strip method, which is 100 times more sensitive than the colloidal gold strip.•The first report of introducing Fe3O4 nanozyme into immunochromatographic strip.•The novel method could be used as a valuable tool for diagnosis of Ebola and other infectious disease.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0956-5663 1873-4235
DOI:	10.1016/j.bios.2015.05.025