3D bioprinting of a corneal stroma equivalent

Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation...

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Veröffentlicht in:Experimental eye research Jg. 173; S. 188 - 193
Hauptverfasser: Isaacson, Abigail, Swioklo, Stephen, Connon, Che J.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier Ltd 01.08.2018
Academic Press
Schlagworte:
3D bioprinting
Bio-ink
Bioartificial Organs
Bioprinting - methods
Collagen
Cornea
Corneal Keratocytes - cytology
Corneal Stroma - cytology
Equipment Design
Humans
Keratocytes
Printing, Three-Dimensional - instrumentation
Tissue Engineering
Tissue Scaffolds
Cornea
3D bioprinting
Bio-ink
Tissue engineering
Keratocytes
Collagen
ISSN:0014-4835, 1096-0007, 1096-0007
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Zusammenfassung:Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. Keratocytes exhibited high cell viability both at day 1 post-printing (>90%) and at day 7 (83%). We established 3D bio-printing to be a feasible method by which artificial corneal structures can be engineered. [Display omitted]
Bibliographie:ObjectType-Article-1
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ISSN:0014-4835
1096-0007
1096-0007
DOI:10.1016/j.exer.2018.05.010