Fast LC-MS/MS analysis of tacrolimus, sirolimus, everolimus and cyclosporin A in dried blood spots and the influence of the hematocrit and immunosuppressant concentration on recovery

We developed a method for the analysis of four immunosuppressants in dried blood spot (DBS) samples to facilitate therapeutic drug monitoring for transplant patients outside the hospital. An 8mm disc from the central part of the DBS was punched, extracted and followed by LC-MS/MS analysis. The metho...

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Bibliographic Details
Published in:	Talanta (Oxford) Vol. 115; pp. 47 - 54
Main Authors:	Koster, Remco A., Alffenaar, Jan-Willem C., Greijdanus, Ben, Uges, Donald R.A.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 15.10.2013
Subjects:	blood blood volume cellulose Chromatography, Liquid Cyclosporin A cyclosporine Cyclosporine - blood Dried blood spot Dried Blood Spot Testing Drug Monitoring Drug Stability Everolimus Hematocrit hospitals Humans hydrogen Immunosuppressive Agents - blood monitoring patients protein binding rapamycin Sirolimus Sirolimus - analogs & derivatives Sirolimus - blood Solid Phase Microextraction Tacrolimus Tacrolimus - blood Tandem Mass Spectrometry therapeutics Cyclosporin A Tacrolimus Sirolimus Everolimus Dried blood spot Hematocrit
ISSN:	0039-9140, 1873-3573, 1873-3573
Online Access:	Get full text
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