Cellular inhibitors with Fv1-like activity restrict human and simian immunodeficiency virus tropism

Many nonhuman primate cells are unable to support the replication of HIV-1, whereas others are nonpermissive for infection by simian immunodeficiency virus from macaques (SIVmac). Here, we show that restricted HIV-1 and SIVmac infection of primate cell lines shares some salient features with Fv1 and...

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Vydáno v:Proceedings of the National Academy of Sciences - PNAS Ročník 99; číslo 18; s. 11914
Hlavní autoři: Cowan, Simone, Hatziioannou, Theodora, Cunningham, Tshaka, Muesing, Mark A, Gottlinger, Heinrich G, Bieniasz, Paul D
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 03.09.2002
Témata:
Animals
Antiviral Agents
Cell Line
HIV-1 - physiology
Humans
Proteins - physiology
Simian Immunodeficiency Virus - physiology
Transcription, Genetic
Tropism
Virus Replication
ISSN:0027-8424
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Shrnutí:Many nonhuman primate cells are unable to support the replication of HIV-1, whereas others are nonpermissive for infection by simian immunodeficiency virus from macaques (SIVmac). Here, we show that restricted HIV-1 and SIVmac infection of primate cell lines shares some salient features with Fv1 and Ref1-mediated restriction of murine retrovirus infection. In particular, the nonpermissive phenotype is most evident at low multiplicities of infection, results in reduced accumulation of reverse transcription products, and is dominant in heterokaryons generated by fusion of permissive and nonpermissive target cells. Moreover, in nonpermissive primate cells, HIV-1 and SIVmac infection is cooperative, and enveloped HIV-1 virus-like particles, minimally containing Gag and protease, abrogate restriction. In African green monkey cells, HIV-1 virus-like particles ablate restrictions to HIV-1 and SIVmac, suggesting that both are restricted by the same factor. Finally, a virus that contains an HIV-1 capsid-p2 domain in an SIVmac background exhibits a tropism for primate cells that is HIV-1-like rather than SIVmac-like. These data indicate the existence of one or more saturable inhibitors that are polymorphic in primates and prevent HIV and SIV infection by targeting the capsid of the incoming lentivirus particle.
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ISSN:0027-8424
DOI:10.1073/pnas.162299499