CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models

This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8+ T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell recept...

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Bibliographic Details
Published in:	STAR protocols Vol. 3; no. 1; p. 101038
Main Authors:	Effern, Maike, Glodde, Nicole, Bawden, Emma, Liebing, Jana, Hinze, Daniel, Tüting, Thomas, Gebhardt, Thomas, Hölzel, Michael
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 18.03.2022 Elsevier
Subjects:	Animals Biotechnology and bioengineering Cancer CD8-Positive T-Lymphocytes Cell Biology CRISPR Disease Models, Animal Epitopes, T-Lymphocyte Flow Cytometry/Mass Cytometry Immunology Immunotherapy, Adoptive - methods Mice Model Organisms Molecular Biology Neoplasms - therapy Protocol Receptors, Antigen, T-Cell - genetics CRISPR Immunology Molecular Biology Biotechnology and bioengineering Flow Cytometry/Mass Cytometry Model Organisms Cell Biology Cancer
ISSN:	2666-1667, 2666-1667
Online Access:	Get full text
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