Analytical development for the assessment of pesticide contaminations in blood and plasma of wild birds: The case of grey partridges (Perdix perdix)

•Methods allowed sensitive detection and quantification of pesticides in birds.•Blood and plasma from grey partridges were appropriated matrices for biomonitoring.•Analyses of both matrices allowed a good assessment of environmental contaminations.•Blood allowed higher levels of detection with easie...

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Veröffentlicht in:Journal of Chromatography A Jg. 1687; S. 463681
Hauptverfasser: Rodrigues, A, Gaffard, A, Moreau, J, Monceau, K, Delhomme, O, Millet, M
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 04.01.2023
Schlagworte:
Analytical Chemistry
Analytical method
Animals
Biochemistry
Biomonitoring
Birds
Blood
Blood samples
Chemistry
Chimie
chromatography
Chromatography, Liquid
Chromatography, Liquid - methods
desorption
Galliformes
GC/MS/MS
General Medicine
LC-MS-MS
moexipril
Organic Chemistry
Perdix perdix
Pesticide contaminations
Pesticide Residues
Pesticide Residues - analysis
Pesticides
Pesticides - analysis
Physical, chemical, mathematical & earth Sciences
Physique, chimie, mathématiques & sciences de la terre
plant protection
Plant protection products
Plasma
Plasma samples
Tandem Mass Spectrometry
Tandem Mass Spectrometry - methods
Wild birds
Plasma
Birds
Biomonitoring
Blood
Pesticides
ISSN:0021-9673, 1873-3778, 1873-3778
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Zusammenfassung:•Methods allowed sensitive detection and quantification of pesticides in birds.•Blood and plasma from grey partridges were appropriated matrices for biomonitoring.•Analyses of both matrices allowed a good assessment of environmental contaminations.•Blood allowed higher levels of detection with easier sampling than plasma. In this study, blood and plasma of grey partridges (Perdix perdix) were analyzed to assess their potential contamination by plant protection products (PPP) and especially pesticide compounds. The group of pesticides selected is composed of a huge variety of compounds. Therefore, in this study, two methods were optimized and validated to analyze 104 compounds including herbicides, insecticides, fungicides and photoprotectors or synergists. Various extraction methods found in the literature were compared and adapted for the extraction of pesticides from blood and plasma. After extraction, samples were concentrated then injected for quantification simultaneously in LC-MS/MS and ATD-GC-MS/MS with an automatic thermal desorption step (ATD). Both LC-MS/MS and ATD-GC-MS/MS analyses were performed using the MRM mode with 2 mass transitions for each compound.The two analytical methods achieved a good linearity for the calibration responses in plasma and blood. Methods allowed sensitive detection and quantification in complex biological matrices such as plasma and blood in both LC and GC. For plasma samples and considering all 104 compounds of the study, the average LOD was 0.005 ng mg−1 in LC-MS/MS and 0.035 ng mg−1 in ATD-GC-MS/MS and the average LOQ was 0.017 ng mg−1 and 0.116 ng mg−1 in LC-MS/MS and ATD-GC-MS/MS respectively. Accordingly, the average LOD for blood samples was 0.011 ng mg−1 in LC and 0.028 ng mg−1 in GC whereas the average LOQ was 0.038 ng mg−1 and 0.094 ng mg−1 in LC-MS/MS and ATD-GC-MS/MS respectively. Those analytical methods were then successfully applied to 70 blood samples and 35 plasma samples.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
scopus-id:2-s2.0-85144636321
ISSN:0021-9673
1873-3778
1873-3778
DOI:10.1016/j.chroma.2022.463681