The Freshwater Cyanobacterium Synechococcus elongatus PCC 7942 Does Not Require an Active External Carbonic Anhydrase

Under standard laboratory conditions, Synechococcus elongatus PCC 7942 lacks EcaASyn, a periplasmic carbonic anhydrase (CA). In this study, a S. elongatus transformant was created that expressed the homologous EcaACya from Cyanothece sp. ATCC 51142. This additional external CA had no discernible eff...

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Bibliographic Details
Published in:Plants (Basel) Vol. 13; no. 16; p. 2323
Main Authors: Kupriyanova, Elena V., Sinetova, Maria A., Gabrielyan, David A., Los, Dmitry A.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 20.08.2024
MDPI
Subjects:
Carbon
Carbon dioxide
Carbon dioxide concentration
carbonate dehydratase
Carbonic anhydrase
Carbonic anhydrases
Cyanobacteria
Cyanothece
Cyanothece sp. ATCC 51142
EcaA
Elongation
Enzymes
freshwater
Genomes
Peptides
Periplasm
physiological role
Physiology
Proteins
Synechococcus elongatus
Synechococcus elongatus PCC 7942
Synechococcus sp. PCC 7942
CO2-concentrating mechanism
NDH-14
cyanobacteria
carbonic anhydrase
physiological role
Cyanothece sp. ATCC 51142
extreme CO2 level
Synechococcus elongatus PCC 7942
EcaA
ISSN:2223-7747, 2223-7747
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Summary:Under standard laboratory conditions, Synechococcus elongatus PCC 7942 lacks EcaASyn, a periplasmic carbonic anhydrase (CA). In this study, a S. elongatus transformant was created that expressed the homologous EcaACya from Cyanothece sp. ATCC 51142. This additional external CA had no discernible effect on the adaptive responses and physiology of cells exposed to changes similar to those found in S. elongatus natural habitats, such as fluctuating CO2 and HCO3− concentrations and ratios, oxidative or light stress, and high CO2. The transformant had a disadvantage over wild-type cells under certain conditions (Na+ depletion, a reduction in CO2). S. elongatus cells lacked their own EcaASyn in all experimental conditions. The results suggest the presence in S. elongatus of mechanisms that limit the appearance of EcaASyn in the periplasm. For the first time, we offer data on the expression pattern of CCM-associated genes during S. elongatus adaptation to CO2 replacement with HCO3−, as well as cell transfer to high CO2 levels (up to 100%). An increase in CO2 concentration coincides with the suppression of the NDH-14 system, which was previously thought to function constitutively.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants13162323